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A Multiplex PCR Method to Define a Narrow Deleted Chromosomal Region of a Tumor Genome - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4117

Category: Genetics and Genomics: Cancer Genetics Protocols: Loss of Heterozygosity Protocols

DNA for analysis is purified using salt precipitation. The method is gentle, limits the breakage of the long chromosomal strands, and avoids the use of phenol and chloroform. It is suitable for use with cultured cells, breast tumor tissue that has been subjected to hormone receptor analysis, and blood samples. The loss of heterozygosity assay is performed using a multiplex PCR, in which one of each primer pair is labeled with a different fluorophor. - [Read A Multiplex PCR Method to Define a Narrow Deleted Chromosomal Region of a Tumor Genome]

Application of Direct Current Electric Fields to Cells and Tissues in vitro - http://www.natureprotocols.com/2006/12/12/application_of_direct_current.php

Category: Cell Biology and Cell Culture

Protocol applies EFs to cells in vitro but has been modified and to use electrotactic chambers to accommodate cells growing in planar culture or in three-dimensional (3D) gels, en bloc tissue cultures in 3D and possible small embryos, such as that from frog and zebra fish. The EF is applied to the cells or tissues cultured in a customer designed electrotactic chamber via agar salt bridges, Steinbergâ€™s solution and Ag/AgCl electrodes. - [Read Application of Direct Current Electric Fields to Cells and Tissues in vitro]

Classic Plant Media - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Classic_Plant_Media.html

Category: Plant Biology Protocols: Plant Tissue Culture

Includes: Chu (N6) basal salt mixture DKW/Juglans basal salt mixture Gamborg's B-5 basal salt mixture Gamborg's B-5 basal salt mixture with minimal organics Hoagland's No. 2 basal salt mixture McCown's woody plant basal salt mixture Murashige and Skoog basal salt mixture (MS) Quoirin and Lepoivre basal salt mixture Schenk and Hildebrandt basal salt mixture White's basal salt mixture - [Read Classic Plant Media]

DNA Electroelution - http://rothlab.ucdavis.edu/protocols/dna-electroelution.html

Category: DNA Protocols: DNA Extraction Protocols: DNA Extraction Polyacrylamide Gels

DNA Electroelution. This protocol describes the purification of DNA by trapping in a high-salt cushion in a "UEA AnalyticalElectroeluter" (IBI). This machine is no longer manufactured, to our knowledge. However, a smiliar device can be easily made from Plexiglas according to the following diagram, taken from Cornel Mulhardt, Molecular Biology and Genomics (2007) Academic Press, p.52: Schimenti Lab - [Read DNA Electroelution]

Enzyme Purification by Salt Ammonium Sulfate Precipitation - http://www.ece.umd.edu/~nsw/ench485/lab6a.htm

Category: Protein Protocols: Protein Precipitation Procols

ENZYME PURIFICATION BY SALT (AMMONIUM SULFATE) PRECIPITATION. Prepared by Nam Sun Wang, Department of Chemical & Biomolecular Engineering. University of Maryland. - [Read Enzyme Purification by Salt Ammonium Sulfate Precipitation]

Induction of Conjugation in Tetrahymena Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4499

Category: Tetrahymena Protozoa Protocols

Mature Tetrahymena cells of opposite mating types are starved under appropriate salt conditions. The mating types are then combined to costimulate through cell-cell interaction. Loose pairs and then firm, irreversible pairs of cells of opposite mating types form. This method consistently results in a high percentage of pairing (usually greater than 80%) and good synchrony. - [Read Induction of Conjugation in Tetrahymena Protocol]

Isolation of T Cells from Synovial Membranes Protocol - http://omrf.ouhsc.edu/~frank/TCELLISO.html

Category: Immunology: Mononuclear Cell Isolation Protocols: T-Cell Isolation Protocols

Protocol for isolation of T cells from synovial membranes. Includes: Initial Cell Isolation; Nonadherent Cell Isolation; Adherent Cell Isolation; Neuraminidase-Treated Sheep Red Blood Cells; Erythrocyte Lysing Solution; Balance Salt Solution (BSS). - [Read Isolation of T Cells from Synovial Membranes Protocol]

Low Salt LB Media Protocol - http://www.thelabrat.com/protocols/LowSaltLB.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Broth Culture Media Protocols

Protocol for low salt LB media. - [Read Low Salt LB Media Protocol]

Nuclear and Splicing Extract Preparation - http://penguin.uchc.edu/~intron/Protocols/NuclearS100%20Extract.html

Category: Protein Protocols: Nuclear Extraction Protocols

S100 extract cytosolic extraction preparation. Buffer A (Hypotonic Buffer), Low and High salt buffer recipes. Brent Graveley - [Read Nuclear and Splicing Extract Preparation]

Plant Tissue Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Media_Preparation.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for plant tissue culture media. Includes: Preparation from Packaged Powder; Preparation from Basal Salt Solutions. - [Read Plant Tissue Culture Media Protocol]

Preparation of RNA from Plant Tissue Using Trizol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4105

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol is based on the standard Trizol protocol for the purification of RNA from animal cells using Trizol (Purification of RNA from Animal Cells using Trizol). In this version, adapted for use with plant tissues, a high-salt isopropanol precipitation step has been added to precipitate RNA selectively, while maintaining polysaccharides and proteoglycans in solution. - [Read Preparation of RNA from Plant Tissue Using Trizol]

Salt solutions and Buffer Calculators - http://molbiol.ru/eng/protocol/01_02.html

Category: Buffers Media, and Solutions

Salt solutions and Buffer Calculators. (Practical Molecular Biology) - [Read Salt solutions and Buffer Calculators]

Staining Chromosomes (G-Banding) Protocol - http://humgen.wustl.edu/hdk_lab_manual/cam/cam4.html

Category: Cytogenetics Protocols

Protocol for staining chromosomes (G-Banding). Includes: Trypsin (1:250) USP Grade; Gurr Buffer Solution: (1 liter); Working Salt Solution: (1 liter); Leishman Stain Solution. - [Read Staining Chromosomes (G-Banding) Protocol]

Staining for Myelin Sheath Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/LFB.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for myelin sheath. Luxol Fast Blue is the alcohol soluble counterpart of the water soluble Alcian Blue. Staining is due to lipoproteins, and the mechanism is one of an acid-base reaction with salt formation; the base of the lipoprotein replaces the base of the dye. - [Read Staining for Myelin Sheath Protocol]

Articles (1-2 of 2)

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

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