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Introduction to the Genetics and Molecular Biology of the Yeast Saccharomyces cerevisiae - http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/index.html

Category: Yeast Protocols: Yeast Genetics Protocols

Introduction of the of genetics and molecular biology of the Yeast Saccharomyces cerevisiae. - [Read Introduction to the Genetics and Molecular Biology of the Yeast Saccharomyces cerevisiae]

Peroxisomes in Saccharomyces cerevisiae - http://mcb.asm.org/cgi/reprint/11/1/510.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Peroxisome Isolation Protocols

To isolate peroxisomes from Saccharomyces cerevisiae of a quality sufficient for in vitro import studies, we optimized the conditions for cell growth and for cell fractionation. Stability of the isolated peroxisomes was monitored by catalase latency and sedimentability of marker enzymes. - [Read Peroxisomes in Saccharomyces cerevisiae]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Single tube confirmation PCR protocol. Saccharomyces Genome Deletion Project. - [Read Single tube confirmation PCR protocol]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: PCR Protocols

Single tube confirmation PCR protocol. Saccharomyces Genome Deletion Project. - [Read Single tube confirmation PCR protocol]

Yeast Growth Rate Cytotoxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/33.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

The proliferation rate of the yeast, Saccharomyces cerevisiae, may be regarded as an overall indicator of the physiological status of the cell. Therefore, the effect of various toxic substances on different cell functions will be reflected by changes in the rate of proliferation. It is possible to determine the toxicity of a test substance simply by measuring cell density. - [Read Yeast Growth Rate Cytotoxicity Test]

Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Category: Yeast Protocols

Plasma membranes are isolated from the yeast Saccharomyces cerevisiae. The cell wall is initially digested by helicase, followed by hypoosmotic lysis and homogenization. Membranes are prepared by subsequent differential centrifugation. The activity of the H+-ATPase is then determined by measuring the amount of inorganic phosphate released from ATP. - [Read Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol]