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Long and Accurate PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4094

Protocol illustrates the rules of successful long PCR: No more than 1 ng of template DNA is used per microliter of PCR in a 100-µl reaction; approximately 0.1 µl of KlentaqLA (not plain Taq) is used per kilobase of target (for targets >10 kb, 1-1.3 µl of enzyme should be used); the Mg++ concentration is considered as the excess over the level of dNTPs. - [Read Long and Accurate PCR Protocol]

Mouse embryonic stem (ES) cell culture - basic procedures - http://www.molgen.mpg.de/~rodent/ES_protocol.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Boris Greber. Important rules for ES cell culture, Signs of differentiation, General tips, ES medium, Thawing ES cells, Freezing, Passaging. - [Read Mouse embryonic stem (ES) cell culture - basic procedures]

Mouse embryonic stem (ES) cell culture - basic procedures PDF - http://www.molgen.mpg.de/~rodent/ES_protocol.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Great Procedures. rules for ES cell culture, differentiation of ES cells, ES medium, Thawing, Passaging, Freezing. Mouse embryonic stem (ES) cell culture - basic procedures. Max Planck Institute for Molecular Genetics. Boris Greber. - [Read Mouse embryonic stem (ES) cell culture - basic procedures PDF]