rotor Protocols

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Beckman Rotor Calculator Tool Converter - http://www.beckman.com/resourcecenter/labresources/centrifuges/rotorcalc.asp

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Beckman Rotor Calculator Tool and Converter. RPM to G's. Rotor calculations. Radius, RCF. - [Read Beckman Rotor Calculator Tool Converter]

Centrifugation Information - http://homepages.gac.edu/~cellab/appds/appd-f.html

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Rotors, Rotor Tubes, Relative Centrifugal Force, Rotor Comparisons. Article - [Read Centrifugation Information]

Centrifuge Safety - http://www.chm.bris.ac.uk/safety/centrifg.htm

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Hazards of Centrifuge use in the laboratory and Operation. Rotor care, and Pre-run safety checks for rotors and centrifuges. - [Read Centrifuge Safety]

Centrifuge Safety - Great Tips - http://www.crscientific.com/centrifuge-safety.html

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Balancing the tubes in the rotor, labeling tubes, and other safety concerns for centrifuge safety. CR Scientific. - [Read Centrifuge Safety - Great Tips]

Purification of Peroxisomes using a Density Barrier in a Swinging-Bucket Rotor - http://www.axis-shield.com/densityhome/optiprep/S10.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Peroxisome Isolation Protocols

Peroxisomes can be purified in iodixanol gradients in high yield (80-90%) with no detectable contamination from any other organelle. This is a property unique to iodixanol because the densities of other organelles, particularly that of mitochondria (approx ρ = 1.14 g/ml) and endoplasmic reticulum (approx ρ = 1.13 g/ml) are much lower than that of peroxisomes (approx ρ = 1.18 g/ml). - [Read Purification of Peroxisomes using a Density Barrier in a Swinging-Bucket Rotor]

Rotor / Adapter Selection Guide - http://www.bdbiosciences.com/discovery_labware/technical_resources/labtools/p42.pdf

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Rotor / Adapter Selection Guide. BD Biosciences. - [Read Rotor / Adapter Selection Guide]

Rotor equilibration without counterbalance - http://www.methods.info/Tips/general_lab/rotor_equilibration.html

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Dr.A.Gratchev - [Read Rotor equilibration without counterbalance]

Subfractionation of Low-Density Lipoprotein (LDL) - http://www.axis-shield.com/densityhome/optiprep/M11.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols

In the routine method described in this protocol, chylomicron-free plasma is adjusted to 12% (w/v) iodixanol and the sample, essentially fills an approx 3 ml tube for a near-vertical rotor. During the centrifugation VLDL, LDL and HDL particles and also plasma proteins migrate from all parts of the sample to their final buoyant density banding position in the self-forming density gradient. - [Read Subfractionation of Low-Density Lipoprotein (LDL)]

Articles (1-5 of 5)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.