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Accumulation and Fixation of Plant Metaphase Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/proaccum.htm

Category: Plant Biology Protocols: Plant Genetics Protocols

For analysis of metaphase chromosomes, any tissue containing dividing cells can be used: Root tips from young seedlings, from newly grown roots at the edge of plant pots or hydroponic culture are all suitable. Alternatively, flower buds, anthers, carpels or leaf or apical meristems can be used. Includes metaphase arresting reagents. - [Read Accumulation and Fixation of Plant Metaphase Chromosomes Protocol]

Adenovirus Protocol - http://www.mc.vanderbilt.edu/root/vumc.php?site=mgcore&doc=4301

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for Adenovirus. - [Read Adenovirus Protocol]

Allium Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/8.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

The Allium test provides a rapid screening procedure for chemicals, pollutants contaminants, etc. which may represent environmental hazards. Root growth inhibition and adverse effects upon chromosomes provide an indication of likely toxicity. - [Read Allium Test]

Allium Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/8.htm

Category: Plant Biology Protocols

Clearing Arabidopsis Non-Ovule Materials With the HCG Solution Protocol - http://www.hybtech.org/Liu/HCG_clearing.htm

Category: Plant Biology Protocols

Protocol can be used for clearing intact non-ovule materials of arabidopsis, which can then be observed under Normarski optics. This is an efficient way to analyse root, seedling even flower development without sectioning. This protocol could also be used for clearing GUS stained material, after chlorophyll is removed by 70% ethanol. - [Read Clearing Arabidopsis Non-Ovule Materials With the HCG Solution Protocol]

Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb19/r01-030.pdf

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana. CL ENCINA1, M CONSTANTIN2 and J BOTELLA2. Plant Molecular Biology Reporter 19: 245â€“248, 2001. - [Read Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana]

Root Transformation of Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4671

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Arabidopsis can be stably transformed using Agrobacterium tumefaciens-mediated transfer of T-DNA. We describe the generation of transgenic plants via root transformation in tissue culture, which can be useful for transforming sterile mutants. - [Read Root Transformation of Arabidopsis Protocol]

Transgene Expression in Regenerated Roots - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4691

Category: Plant Biology Protocols: Plant Genetics Protocols

This procedure, which uses a root transformation protocol, provides a rapid method for assessing gene expression in Arabidopsis roots. It is useful for testing promoter:reporter gene constructs, for expressing genes, the overexpression of which is lethal in whole plants, and for transforming the roots of plants that are recalcitrant to conventional transformation techniques. The protocol has been used successfully with Ws, No-0, and RLD ecotypes. - [Read Transgene Expression in Regenerated Roots]