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Absorbance Assay 205 nm - http://www.ruf.rice.edu/~bioslabs/methods/protein/abs205.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Absorbance assay at 280 nm. This method is just as convenient as for absorbance at 280 nm. It may be preferred if there is excessive contamination by nucleic acids, since nucleic acids absorb very little radiation at 205 nm. Setting the wavelength is a bit tricky since 205 nm is right on the shoulder of the protein peak. - [Read Absorbance Assay 205 nm]

Absorbance Assay 280 nm - http://www.ruf.rice.edu/~bioslabs/methods/protein/abs280.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Absorbance assays are fast and convenient, since no additional reagents or incubations are required. No protein standard need be prepared. The assay does not consume the protein. The relationship of absorbance to protein concentration is linear. Because different proteins and nucleic acids have widely varying absorption characteristics there may be considerable error, especially for unknowns or protein mixtures. - [Read Absorbance Assay 280 nm]

Agrobacterium-Mediated Transformation in Rice Protocol - http://www.natureprotocols.com/2007/01/18/a_protocol_for_agrobacteriumme.php

Category: Cytogenetics Protocols

Protocol for Agrobacterium-mediated transformation in rice. Agrobacterium-mediated rice transformation method that is applicable to easily cultured varieties in addition to elite japonica varieties that are more difficult to culture. Using this method, transgenic rice plants can be obtained in about 2–3 months with a transformation frequency of 30–50%, both in easily cultured varieties and recalcitrant elite japonica rice. - [Read Agrobacterium-Mediated Transformation in Rice Protocol]

Amplification of Insert End Sequences from BACs Clones by Thermal Asymmetric Interlaced PCR - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb16/16175-2.pdf

Category: BACs Bacterial Artificial Chromosome Protocols

Protocol presents the amplification of insert end sequences from bacterial artificial chromosome clones using TAIL-PCR. The amplified products are suitable as probes for chromosome walking and genome mapping and as templates for direct sequencing. The protocol has been used in rice genome studies. - [Read Amplification of Insert End Sequences from BACs Clones by Thermal Asymmetric Interlaced PCR]

Analysis of Protein Gels (SDS-PAGE) - http://www.ruf.rice.edu/~bioslabs/studies/sds-page/gellab3.html

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Bradford Protein Assay - http://www.ruf.rice.edu/~bioslabs/methods/protein/bradford.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

A detailed and well thought out Bradford protein assay page using a spectrophotometer. Includes information such as the Bradford assay is very fast and uses about the same amount of protein as the Lowry assay, comments, procedural steps, equipment used and more. - [Read Bradford Protein Assay]

Detection and Measurement of Radioactivity - http://www.ruf.rice.edu/%7Ebioslabs/methods/radioisotopes/rad1.html

Category: Radioactivity

Radioactive Decay, Methods of detection, Liquid scintillation counting, Background and Quenching, The scintillation counter printout. David R. Caprette, Rice Univ. - [Read Detection and Measurement of Radioactivity]

Determination of the Extinction Coefficient for a Protein of Unknown Concentration - http://www.ruf.rice.edu/~bioslabs/methods/protein/absext.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Determination of the Extinction Coefficient for a Protein of Unknown Concentration. The concentration can be determined for a solution of a pure protein with unknown extinction coefficient. - [Read Determination of the Extinction Coefficient for a Protein of Unknown Concentration]

General Overview of Histology - http://www-bioc.rice.edu/bios576/histology/histology.htm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation must convert the soluble contents of the cell (ie proteins, carbohydrates, ... http://www.protocol-online.org/prot/Histology/Fixation/ ...General Overview of Histology and Tissue Fixation. BIOS 576. Rice. - [Read General Overview of Histology]

Introduction to SDS-PAGE - http://www.ruf.rice.edu/%7Ebioslabs/studies/sds-page/gellab2.html#gelprep

Category: Protein Protocols: Protein Electrophoresis

A great introduction to SDS-PAGE. David R. Caprette, Rice University. - [Read Introduction to SDS-PAGE]

Ligation with GUS vector and Transformation - http://www.owlnet.rice.edu/~bios311/bios311/bios312/312day3.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

Ligation with GUS vector and Transformation. Chemical Transformation of E. coli. Ligation Procedure for LigaFastâ„¢ Rapid DNA Ligation System (Promega). B. Beason, Rice University. - [Read Ligation with GUS vector and Transformation]

Light Microscopy - http://www.ruf.rice.edu/%7Ebioslabs/methods/microscopy/microscopy.html

Category: Molecular Imaging: Light Microscopy

Types of light microscopy, Bright Field Microscopy, Using a bright field microscope, mounting on slides, adjusting the microscope, Care of the microscope, When to use bright field microscopy. David R. Caprette. Rice University. - [Read Light Microscopy]

List of Protein Assays - http://www.ruf.rice.edu/~bioslabs/methods/protein/protein.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Find a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/amount of sample needed, subjective comments on accuracy and convenience, and major interfering agents. Procedural details, equipment requirements, and references are outlined in the individual assay documents. - [Read List of Protein Assays]