rhodamine Protocols

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Preparation and Microinjection of x-Rhodamine-Labeled Vimentin Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4676

Category: Cytoskeleton Protocols: Intermediate Filaments Protocols

Intermediate filaments (IF) are major cytoskeletal systems of vertebrate and many nonvertebrate cells whose expression is cell-type specific and developmentally regulated. This protocol describes the x-rhodamine labeling of one type of IF, vimentin, and a method for microinjection of the labeled vimentin into cultured cells. IF dynamics can then be examined with fluorescence microscopy. - [Read Preparation and Microinjection of x-Rhodamine-Labeled Vimentin Protocol]

Protocol for Motor Polarity Using Fluorescent Microtubules - http://www.proweb.org/kinesin/Methods/Fluorescent_MT.html

Category: Cell Biology and Cell Culture

Protocol for motor polarity using fluorescent microtubules using the materials: Rhodamine-tubulin (Molecular Probes),NEM- tubulin, PC-tubulin, MgÂ·GTP, MgCl2 and Glycerol. - [Read Protocol for Motor Polarity Using Fluorescent Microtubules]