retroviruses Protocols

Category: Cell Biology and Cell Culture

Purification protocols of the viruses: HIV-1, Lassa virus, oncornavirus and other retroviruses. Protocol uses an iodixanol gradient in a sedimentation velocity mode to purifyHIV-1 virions without affecting the infectivity of the virus. In rate-zonal iodixanol gradients the HIV-1 was effectively separated both from Vif and from the microvesicles. - [Read M5 Velocity (rate zonal) gradients for purification and assembly analysis of viruses.]

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Use of “293-GPG” cells to generate amphotropic retroviruses, plus infection protocol. - [Read Use of “293-GPG” cells to Generate Amphotropic Retroviruses, plus Infection Protocol]