resources Protocols

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Balancing Rotors, Tips on Centrifuging. Cautions for centrifuge usage. - [Read Centrifuge Article and Tips]

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol for chloroplast DNA isolation. - [Read Chloroplast DNA Isolation Protocol]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for chromosome preparation and Fluorescence in-situ hybridization (FISH). Chromosome preparation and Fluorescence in-situ hybridization (FISH). - [Read Chromosome Preparation and Fluorescence In-Situ Hybridization (FISH) Protocol]

Category: RNA Protocols: RT-PCR and cDNA synthesis

Detecting DNA Contamination in RT-PCR Qiagen. - [Read Detecting DNA Contamination in RT-PCR Qiagen.]

Category: Pharmacology and Toxicology Protocols

Protocol for the determination of IC50. - [Read Determination of IC50 Protocol]

Category: E Coli Protocols: E coli Nucleic Acid Protocols

E.coli total RNA labeling protocol for high density oligonucleotide array. Includes: RNA Preparation; Digest RNA and Purification of cDNA; Purify cDNA with Qiaquick PCR purification kit; cDNA Fragmentation and end labeling; Labeling with Terminal Transferase. - [Read E.coli Total RNA Labeling Protocol for High Density Oligonucleotide Array]

Category: Microarray Protocols: Microarray DNA Labeling Protocols

Protocol for e.coli total RNA labeling for high density oligonucleotide array. - [Read E.coli Total RNA Labeling Protocol for High Density Oligonucleotide Array]

Category: E Coli Protocols: E coli Nucleic Acid Protocols

Protocol for E coli total RNA labeling for spotted microarray. Includes: RNA Preparation; Labeling; Clean up Labeled Probes. - [Read E.coli Total RNA Labeling Protocol for Spotted Microarray]

Category: Histology Protocols: Tissue Sample Sectioning

PREPARATION OF PLANT TISSUE FOR LASER MICRODISSECTION. Schnable Labs, Plant Genomics. - [Read FROZEN SECTIONING PROTOCOL Plant Tissue PDF]

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Fugene Transfection Protocol PDF. Meyer Lab Standford. - [Read Fugene Transfection Protocol PDF]

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Protocol for genomic DNA labeling. - [Read Genomic DNA Labeling Protocol]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription with SP6 polymerase protocol. - [Read In Vitro Transcription with SP6 Polymerase Protocol]

Category: Immunology: Mononuclear Cell Isolation Protocols

Mononuclear phagocyte progenitor cells derived from femoral and tibial bone marrow are propagated in the presence of M-CSF. This macrophage growth factor is secreted by L929 cells and is used in the form of L929 cell conditioned medium. The progenitor cells proliferate and differentiate through monoblast, promonocyte and monocyte stages before maturing to macrophages. At this time the cells become firmly adherent to the culture vessel. - [Read Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol]

Category: Plant Biology Protocols: Plant Tissue Culture

Cryopreservation protocols for different kinds of tissue of banana ... Teaching Plant Tissue Culture: University of Liverpool ... - [Read Kitchen Culture Kits - Plant Tissue Culture Resources]

Category: Histology Protocols: Tissue Sample Sectioning

FIXATION, DEHYDRATION, PARAPLAST INFILTRATION AND SECTIONING PROTOCOL WITH TISSUE COLLECTION PROCEDURE USING THE PALM LASER MICRODISSECTION SYSTEM. Schnable Labs, Plant Genomics. - [Read Paraffin Sectioning for LCM Plant Tissue]

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

PCR GENOTYPING PROTOCOL. 1) Cut toes of mice at approx. ten days of age and record sex, color, and strain. ... Back to Mouse Genotyping Resources. - [Read PCR GENOTYPING PROTOCOL]

Category: Yeast Protocols: Yeast Genetics Protocols

When more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt by interaction mating. In this protocol one strain is transformed with library DNA and the transformants are collected and frozen in aliquots. - [Read Performing a Hunt by Interaction Mating Protocol]

Category: E Coli Protocols: E coli Genetics Protocols

Protocol for precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli. Describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements. - [Read Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in E coli Protocol]

Category: Plant Biology Protocols: Plant Genetics Protocols

Describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements. - [Read Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in Escherichia Coli]

Category: Cell Biology and Cell Culture

Matirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens, laminin, and proteoglycans)but also matrix degrading enzymes/their inhibitors and growth factors. Invasion of tumor cells into Matrigel has been used to characterize involvement of ECM receptors and matrix degrading enzymes which play roles in tumor progression. - [Read Protocol for Matrigel Invasion Assays]

Category: siRNA and RNAi Protocols

RNAi Interference Resources - Ambion. Introduction, product database for siRNA and RNAi. Ambion. - [Read RNAi Interference Resources - Ambion]

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for spotted microarray hybridization and scan. Includes: Setup Hybridization; Wash Slides; Scan Slides. - [Read Spotted Microarray Hybridization and Scan Protocol]

Category: Buffers Media, and Solutions

Theory: How to Make Simple Solutions and Dilutions. Information on dilution making. - [Read Theory: How to Make Simple Solutions and Dilutions]

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for transposon mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon Mediated Mutagenesis Protocol]

Category: Bacteria Genetics Protocols

Protocol for transposon-mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon-Mediated Mutagenesis Protocol]