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Antibiotic and Selection Reagent Usage for Resistant E. coli Cells PDF - http://www.atcc.org/pdf/AntibioticTable.pdf

Category: Microbiology: Antibiotics Concentration in Media LB

Antibiotics, ampicillin, choramphenicol, G418, X-Gal and IPTG also stock concentrations. ATCC. - [Read Antibiotic and Selection Reagent Usage for Resistant E. coli Cells PDF]

Holzer's Stain Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/HOLZER.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols

An appropriate term for glial fibers is 'nerve glue', because they provide the internal support of the central nervous system. There are four types of glial cells: astrocytes, oligosendroglia,microglia, and ependymal cells. The glia fibers are stained with crystal violet which are resistant to the aniline-chloroform differentiating solution. - [Read Holzer's Stain Protocol]

Kanamycin Selection of Transformed Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4669

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

The most commonly used markers for selection of transgenic Arabidopsis are resistance to the antibiotic kanamycin and to the herbicide glufosinate ammonium. Resistance to kanamycin is conferred by a bacterial gene encoding the enzyme neomycin phosphotransferase (NPT). In this protocol, kanamycin-resistant seedlings are selected on solid medium. - [Read Kanamycin Selection of Transformed Arabidopsis Protocol]

PCR Analysis of Agrobacterium Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4667

Category: Bacteria Genetics Protocols

Protocol describes a method to determine the presence of plasmid DNA in an Agrobacterium culture. Compared to selection of transformed Agrobacterium, which can be ambiguous and normally takes several days for resistant colonies to appear, the approach described here is both rapid and accurate. - [Read PCR Analysis of Agrobacterium Protocol]

Preparation of siRNAs from Drosophila Embryo Extracts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4325

Category: RNAi Technology Protocols: RNAi Drosophila Protocols

siRNAs produced upon the addition of dsRNA to Drosophila embryo extract are enriched in a micrococcus-nuclease-resistant fraction. After proteinase K treatment and dephosphorylation with calf intestinal phosphatase, these siRNAs mediate efficient RNAi in vitro. - [Read Preparation of siRNAs from Drosophila Embryo Extracts Protocol]

Storage of Sera Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4279

Category: Antibody Protocols: Antibody Handling Protocols

This protocol describes the methods of storage for antibody-containing sera. Antibodies are resistant to a broad range of mildly denaturing conditions, so long-term storage is relatively easy. - [Read Storage of Sera Protocol]

Transformation of Tetrahymena thermophila by Electroporation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4502

Category: Tetrahymena Protozoa Protocols

Protocol describes a method for transformation of the Tetrahymena using electroporation. The vector is electroporated into cells after mating, where it is incorporated into the DNA of developing macronuclei. Because T. thermophila can be propagated indefinitely without conjugation, transformation of the macronucleus provides a way to obtain stable somatic transformants. DNA vectors transformed using this protocol include those containing drug-resistant versions of Tetrahymena genes. - [Read Transformation of Tetrahymena thermophila by Electroporation Protocol]

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Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.