User Panel

My Panel

Bookmark Science Articles

resin Protocols

Search results for: resin

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Affinity Purification of Interacting Proteins from Cell Lysates Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4593

Category: Chromatography Protocols: Affinity Chromatography Protocols

Recombinant protein or a chemically synthesized bioactive fragment is immobilized on resin and used as a probe to capture interacting proteins directly from a cell extract. Affinity-purified proteins are fractionated by gel electrophoresis and visualized by Coomassie staining. Proteins that interact specifically are identified by comparing this gel profile to one obtained from cell lysates passed over a control resin lacking the immobilized probe protein. - [Read Affinity Purification of Interacting Proteins from Cell Lysates Protocol]

Coupling Peptides To Resin For Affinity Purification - Claire Walczak. - http://mitchison.med.harvard.edu/protocols/ab3.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Coupling Peptides To Resin For Affinity Purification - Claire Walczak. - [Read Coupling Peptides To Resin For Affinity Purification - Claire Walczak.]

Directed Orientation of Antibodies during Preparation of Antibody Affinity Resin Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4204

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Protocol describes the directed coupling of antibody to protein G-agarose via its Fc domain, and subsequent covalent cross-linking of the complex using dimethyl pimelimidate (DMP). - [Read Directed Orientation of Antibodies during Preparation of Antibody Affinity Resin Protocol]

DNA Affinity Chromatography Using Gravity Flow - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4206

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

DNA Affinity Chromatography, DNA affinity chromatography can be a low-tech method using gravity flow at 4Â°C, a disposable chromatography column, and DNA affinity resin prepared in the laboratory (see Preparation of a DNA Affinity Column). Include 10-20% glycerol and 0.025-0.1% NP-40 in the column buffers to suppress losses due to nonspecific adsorption of protein to surfaces. Load the protein in a buffer that is compatible with binding of the protein to its target site. Keith Brocklehurst et al - [Read DNA Affinity Chromatography Using Gravity Flow - Subscription Required]

ELECTRON MICROSCOPY (EM) protocols and methods - http://www.bristol.ac.uk/vetpath/cpl/emtechs.htm

Category: Molecular Imaging: Electron Microscopy Protocols

E.M. processing schedule - epoxy resin E.M. processing schedule - acrylic resin Primary fixative and buffers Veterinary Pathology. ELECTRON MICROSCOPY (EM) protocols and methods - [Read ELECTRON MICROSCOPY (EM) protocols and methods]

Electron Microscopy Protocols Yale - http://cellserv.med.yale.edu/imaging/ccmi/elect_protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Protocol 2 - Embedding of cell monolayer in epoxy resin. Protocol 3 - Embedding of scraped cells ... Welcome to the new CCMI: Electron Microscopy Website. ... Yale Protocols. - [Read Electron Microscopy Protocols Yale]

Preparation of DNA Affinity Resin - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4205

Category: Chromatography Protocols

This protocol describes the preparation of concatamerized oligonucleotides and their coupling to cyanogen-bromide-activated Sepharose. The procedure uses a commercially activated resin, which can be purchased as a lyophilized powder. Keith Brocklehurst et al. - [Read Preparation of DNA Affinity Resin - Subscription Required]

Protein Purification: Gel Filtration, Affinity and Hydrophobic resins; Preparation of Resin, Plumbin - http://wine1.sb.fsu.edu/bch5425/lect31/lect31.htm

Category: Protein Protocols: Protein Purification and Identification

Protein Purification: Gel Filtration, Affinity and Hydrophobic resins; Preparation of Resin, Plumbing. Blaber Lecture. - [Read Protein Purification: Gel Filtration, Affinity and Hydrophobic resins; Preparation of Resin, Plumbin]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

Purification of Histidine-tagged Proteins by Immobilized Ni2+ Absorption Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4088

Category: Chromatography Protocols: Affinity Chromatography Protocols

Recombinant proteins engineered to have a polyhistidine tail at either the carboxyl or amino terminus can easily be purified in one step by affinity chromatography on a resin carrying chelated nickel ions. Chromatography can be carried out in column or batch formats. After unbound proteins are washed away, the target protein is eluted using imidazole, which typically preserves the antigenic and functional features of the protein. - [Read Purification of Histidine-tagged Proteins by Immobilized Ni2+ Absorption Chromatography Protocol]

Purification of Maltose-binding Fusion Proteins by Affinity Chromatography on Amylose Resin Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4087

Category: Chromatography Protocols: Affinity Chromatography Protocols

Foreign proteins fused to maltose-binding protein can be readily purified to near homogeneity by affinity chromatography on resins containing cross-linked polysaccharides such as amylose. - [Read Purification of Maltose-binding Fusion Proteins by Affinity Chromatography on Amylose Resin Protocol]

Removal of Ethidium Bromide from DNA by Ion-exchange Chromatography Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/2/pdb.prot3915

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for removal of ethidium bromide from DNA by ion-exchange chromatography. Ethidium bromide is removed from DNA by chromatography through a cation-exchange resin. - [Read Removal of Ethidium Bromide from DNA by Ion-exchange Chromatography Protocol]

Resin Embedding for High Resolution Morphology Protocol - http://www.nottingham.ac.uk/pathology/protocols/resinthins.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for resin embedding for high resolution morphology. - [Read Resin Embedding for High Resolution Morphology Protocol]

Resin Processing of Bone Protocol - http://www.nottingham.ac.uk/pathology/protocols/resbonecut.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for resin processing of bone. Includes: FIXATION; PROCESSING; EMBEDDING; RELEASING BLOCK FROM GLASS CONTAINER; SECTIONING. - [Read Resin Processing of Bone Protocol]

Resin Staining Protocols - http://www.nottingham.ac.uk/pathology/protocols/resinstain.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocols for resin staining. Includes: HAEMATOXYLIN AND EOSIN; GILLS DOUBLE STRENGTH HAEMATOXYLIN; PERIODIC ACID SCHIFF (PAS). - [Read Resin Staining Protocols]

SECTIONING RESIN EMBEDDED TISSUE - http://www.nottingham.ac.uk/pathology/protocols/resinsect.html

Category: Histology Protocols: Tissue Sample Sectioning

SECTIONING RESIN EMBEDDED TISSUE. MAKING LATTA-HARTMANN KNIVES and Sectioning Protocols for Histology. University of Nottingham Medical School Division of Histopathology. James Lowe. - [Read SECTIONING RESIN EMBEDDED TISSUE]

Sectioning Resin Embedded Tissue Protocol - http://www.nottingham.ac.uk/pathology/protocols/resinsect.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for sectioning resin embedded tissue. Includes: MAKING LATTA-HARTMANN KNIVES; SECTIONING. - [Read Sectioning Resin Embedded Tissue Protocol]

Staining Resin Sections of Bone Protocol - http://www.nottingham.ac.uk/pathology/protocols/resbonestain.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for staining resin sections of bone. Includes: VON KOSSA/VAN GIESON; GOLDNER TRICHROME (MODIFICATION); TOLUIDINE BLUE; PERLS PRUSSIAN BLUE; SECTIONS LABELLED WITH TETRACYCLINE; FLATTENING FREE-FLOATING SECTIONS; MOUNTING FREE-FLOATING SECTIONS; CLEANING SLIDES. - [Read Staining Resin Sections of Bone Protocol]

Articles (1-3 of 3)

Purifying DNA Fragments from Agarose Gels Alternate Protocol

An alternate protocol for purifying DNA fragments from agarose gels.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

[1-3]