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Adenovirus DNA Replication Assay Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/AdDNAreplication.html

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol describes large fragment DNA replication by Adenovirus proteins using TP-DNA as template. - [Read Adenovirus DNA Replication Assay Protocol]

Delivery of dsRNA into Plants by VIGS Methodology - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4327

Category: Plant Biology Protocols: Plant Genetics Protocols

Virus-induced gene silencing (VIGS) uses a virus to deliver a sequence from a gene of interest into a host plant. The virus carrying the fragment of the gene of interest must be capable of replication if dsRNA is to be produced. One or two leaves are inoculated with Agrobacterium strains carrying the VIGS vector possessing the gene fragment. The virus then replicates and spreads throughout the plant, mediating silencing. - [Read Delivery of dsRNA into Plants by VIGS Methodology]

Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol - http://www.natureprotocols.com/2006/10/27/fluorescence_in_situ_hybridiza.php

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization (FISH) for DNA replication origins. Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique used for the detection of specific chromosomal rearrangements and applicable to many different specimen types. FISH is widely used for several diagnostic applications. - [Read Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol]

Lineage Analysis Using Retroviral Vectors - http://genetics.med.harvard.edu/~cepko/lineage/

Category: Developmental Biology

Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues. An overview of the relevant aspects of the retroviral life cycle, and the strategies and current methods in use at their laboratory are described. - [Read Lineage Analysis Using Retroviral Vectors]

Lineage Analysis Using Retroviral Vectors Protocol - http://genetics.med.harvard.edu/~cepko/lineage/

Category: Cloning Protocols: Vector Protocols

Protocol describes the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues. - [Read Lineage Analysis Using Retroviral Vectors Protocol]

MICROARRAY cDNA CLONE GROWTH AND TEMPLATE - http://pga.tigr.org/sop/M001_1a.pdf

Category: DNA Microarray Protocols

This protocol describes clone handling, plate replication, and DNA template preparation in a 96 well format. Hasseman. TIGR Microarray Protocols - [Read MICROARRAY cDNA CLONE GROWTH AND TEMPLATE]

Replication Timing by Comparative Hybridization Protocol - http://fangman-brewer.genetics.washington.edu/comparative_hyb.html

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing by comparative hybridization. - [Read Replication Timing by Comparative Hybridization Protocol]

Replication Timing by Density Transfer Protocol - http://fangman-brewer.genetics.washington.edu/density_transfer.html

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing by density transfer. - [Read Replication Timing by Density Transfer Protocol]

Replication Timing Using Transient Hemimethylation Protocol - http://fangman-brewer.genetics.washington.edu/hemimethylation.html

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing using transient hemimethylation. Includes: Strain construction; Protocol for methylation timing; Quantitation. - [Read Replication Timing Using Transient Hemimethylation Protocol]

Transient Transfection Into 293T Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_293T.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1A gene product. 293T is a derivative which also express SV40 large T antigen, allowing episomal replication of plasmids containing the SV40 origin and early promoter region. They (both) have the unusual property of being highly transfectable. - [Read Transient Transfection Into 293T Cells Protocol]

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Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.