reliable Protocols

Category: Cell Biology and Cell Culture: Endothelial Cell Protocols

Traditional animal models to quantify the degree of blood vessel formation are being replaced by cell culture assays that are easier to set up, statistically reliable and can be automated in a drug screening laboratory. These assays rely on the endothelial cells’ ability to form distinct blood-vessel-like tubules in an extracellular matrix where they can subsequently be visualized by fluorescence microscopy. - [Read An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis]

Category: Immunology: Mononuclear Cell Isolation Protocols: B Lymphocyte Isolation Protocols

Protocol describes the purification of mouse T cells, B cells, and T cell subsets using magnetic bead separation. Isolation of cell subsets using magnetic beads is quick, simple, and reliable and can result in high yields of very pure cells. - [Read Fractionation of T and B Cells Using Magnetic Beads Protocol]

Category: Immunology: Mononuclear Cell Isolation Protocols: T-Cell Isolation Protocols

Protocol describes the purification of mouse T cells, B cells, and T cell subsets using magnetic bead separation. Isolation of cell subsets using magnetic beads is quick, simple, and reliable and can result in high yields of very pure cells. - [Read Fractionation of T and B Cells Using Magnetic Beads Protocol]

Category: Immunohistochemistry Protocols

Describes two methods for using the immunoperoxidase reaction to localize antigens at the electron microscope level; one for adherent cultured cells and one for tissue sections. The reaction conditions are first optimized at the light microscope level and then adapted for EM level observation. These methods allow for reliable detection of antigens at the cell surface, within the cell, and especially in membrane bounded organelles. - [Read Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues]

Category: Fungi Protocols: Fungi Genetics Protocols

Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction. The advantages of this method include: 1. The tissue is grown and lyophilized in the same tube, which facilitates the simultaneous analysis of many segregants. 2. Only one extraction step is necessary. 3. The markers are scored by gel electrophoresis, thereby bypassing Southern analysis. - [Read Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR]

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

A reliable method to prepare plate lysates and to recover infectious bacteriophages by elution from the top agar. - [Read Preparing Stocks of Bacteriophage {lambda} by Plate Lysis and Elution Protocol]

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

The protocol given makes the method of in situ hybridization easier, faster, more reliable, and available to anyone who can operate a microscope. Includes: Labeling the hybridization probe; Preparation and denaturation of polytene chromosomes from Drosophila, Chironomus, or other species; Hybridization and detection. - [Read Protocol for Nonradioactive In Situ Hybridization to Polytene Chromosomes with a DIG-labeled DNA]

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Protocol for rapid and reliable preparation of total cell lysates from fission yeast. Method for successful and reproducible extraction of proteins from S. pombe cells. - [Read Rapid and Reliable Preparation of Total Cell Lysates from Fission Yeast Protocol]

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

A messy but reliable technique that works well for DNAs ranging in size from 200 bp to >50 kb. - [Read Recovery of DNA from Agarose and Polyacrylamide Gels: Electroelution into Dialysis Bags Protocol]

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols

A messy but reliable technique that works well for DNAs ranging in size from 200 bp to >50 kb. - [Read Recovery of DNA from Agarose and Polyacrylamide Gels: Electroelution into Dialysis Bags Protocol]

Category: Protein Protocols: Protein Precipitation Procols

Simple and Reliable Method To Precipitate Proteins from Bacterial Culture Supernatant. Randolph B. Caldwell et al., AEM ASM. - [Read Simple and Reliable Method To Precipitate Proteins from Bacterial Culture Supernatant PDF]

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol describes a simple procedure for DNA fragment isolation and purification. The resulting DNA fragment can then. be used for microinjection to produce transgenic mice - [Read Simple, Reliable Steps for DNA Fragment Isolation and Purification Protocol]

Category: Yeast Protocols: Yeast Protein Protocols

Protocol is a simple, reliable method for the preparation of yeast protein extracts for analysis by polyacrylamide gel electrophoresis (PAGE) and Western blotting. - [Read Yeast Protein Extracts Protocol]