related Protocols

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Peroxisome Isolation Protocols

Peroxisomes of higher eukaryotes, glycosomes of kinetoplastids, & glyoxysomes of plants are related microbody organelles that perform differing metabolic functions tailored to their cellular environments. The close evolutionary relationship of these organelles is most clearly evidenced by the conservation of proteins involved in matrix protein import and biogenesis. glycosome can be viewed as an offshoot of the peroxisomal lineage with additional metabolic functions, specifically glycolysi - [Read Biogenesis and Function of Peroxisomes and Glycosomes]

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol and information related to C. elegans integrating extrachromasomal arrays. - [Read C. elegans Integrating Extrachromasomal Arrays Protocol]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti-sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA transcript using reverse transcriptase and labeled through incorporation of a fluorescent-labeled dUTP nucleotide base. This procedure provides rapid detection of low abundance mRNA messages that can be related to other cellular.... - [Read Fluorescent In Situ Transcription in Cells and Tissues Protocol]

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Guide For Identifying And Correcting Common Cell Growth Problems. Corning. Surface Treatment Process, Problems Related to Technique, Problems Related to Incubators, Problems Related to Culture Media, Problem Solving Suggestions. - [Read Guide For Identifying And Correcting Common Cell Growth Problems]

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Protocols for cell fixation and tissue fixation. HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block techniques. Embedding Tissue Into Frozen Blocks. Fixing Cells with Fresh Paraformaldehyde. Preparation of Frozen Sections. Immunohistochemistry. Paraffin sections and Frozen sections. DERC Univ. Colorado PDF. - [Read HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF]

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Using confocal laser-scanning microscope & GFP fusion proteins in time-lapse imaging to visualize the behavior of organelles and to track membrane-bound transport intermediates that bud off from organelles. Practical issues related to construction & expression of GFP fusion proteins are discussed. Essential for optimizing the brightness and expression levels of GFP fusion proteins so that intracellular membrane-bound structures containing these fusion proteins can be readily visualized. - [Read Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell. - [Read Methods for Synchronizing Cells at Specific Stages of the Cell Cycle]

Category: Molecular Biology Protocols: Phage Protocols and Methods

Paul's Phage Protocols. Many phage related protocols - [Read Paul's Phage Protocols]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

The basis of this test is that a cytotoxic chemical (regardless of site or mechanism of action) will interfere with the normal motility of the protozoan, Tetrahymena thermophila, in culture.  The degree of interference of motility as compared to control cultures, related to the concentration of the test compound, provides an indication of toxicity. - [Read Tetrahymena Thermophila Ocular Irritancy Test]

Category: Microscopy Protocols: Optical Microscopy Protocols

TIRM is a optical technique for monitoring the instantaneous separation distance between a microscopic sphere & a flat plate. Changes in distance as small as 1 nm can be detected. Includes information on: Scattering Intensity I is Related to Elevation h ; apparatus. - [Read Total Internal Reflection Microscopy]

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]