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Aminopeptidase M - http://prowl.rockefeller.edu/recipes/nproteas/aminom.htm

Category: Mass Spectrometry Protocols

Aminopeptidase M protocol and properties. PROWL - [Read Aminopeptidase M]

Carboxypeptidase-Y C-terminal sequencing - http://prowl.rockefeller.edu/recipes/cproteas/carboxyy.htm

Category: Mass Spectrometry Protocols

Carboxypeptidase-Y C-terminal sequencing and sequencing ladder protocol method. PROWL - [Read Carboxypeptidase-Y C-terminal sequencing]

Chromatin Immunoprecipitation (ChIPs) Protocol - http://genomecenter.ucdavis.edu/farnham/farnham/protocols/chips.html

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

IP Wash buffer recipes and protocol for ChIP Assay. Protocol works with NIH 3T3, Friend, HeLa, Raji and CHO cells.. Farnham Lab - [Read Chromatin Immunoprecipitation (ChIPs) Protocol]

Common Buffers - http://wheat.pw.usda.gov/~lazo/methods/lazo/buffer.html

Category: Buffers Media, and Solutions

Recipes for commonly used buffer solutions. (Gerard R. Lazo) - [Read Common Buffers]

Common DNA solutions. - http://www.rickhershberger.com/bioactivesite/lab/recipes.htm

Category: Buffers Media, and Solutions: Buffer Recipes for DNA Work

Common DNA solutions.Dr. Rick Hershberger - [Read Common DNA solutions.]

Derivation of Mouse Embryonic Fibroblasts (MEF) Part I PDF - http://www.wicell.org/uploads/media/Protocols_Part_1_05.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

WiCell Research Institute. Recipes, Frequently Asked Questions, Mouse Embryo Extraction and Tissue, Harvesting Mouse Embryonic Fibroblasts (MEF)from mice. - [Read Derivation of Mouse Embryonic Fibroblasts (MEF) Part I PDF]

Detailed NF-kB EMSA Protocol - http://www.celldeath.de/apometh/emsa.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: NF-kB EMSA Protocols

Detailed NF-kB EMSA Protocol. Includes Nuclear extract preparation, Labeling of the oligonucleotide probe: T4 Polynucleotide Kinase Reaction, Supershift assay to identify NF-kB, and recipes for EMSA buffers. Celldeath.de - [Read Detailed NF-kB EMSA Protocol]

Detection of Recombinant Proteins by ELISA - http://omrf.ouhsc.edu/~frank/ELISA.html

Category: Recombinant Protein Protocols

ELISA detection of preparation of bacterially expressed protein. ELISA REAGENT RECIPES. Frank Lab. - [Read Detection of Recombinant Proteins by ELISA]

Drosophila Cornmeal, Dextrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/dextrosefood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, dextrose and yeast medium. - [Read Drosophila Cornmeal, Dextrose and Yeast Medium Protocol]

Drosophila Cornmeal, Molasses and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, molasses and yeast medium. - [Read Drosophila Cornmeal, Molasses and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/harvardfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose and yeast medium. - [Read Drosophila Cornmeal, Sucrose and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/caltechfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose, dextrose, yeast and 2-acid medium. - [Read Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol]

Drosophila Fly Food Prep Method Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/method_press_n_seal.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila fly food prep method. - [Read Drosophila Fly Food Prep Method Protocol]

Drosophila Hard Agar Medium for Egg Collection Plates Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/hardagar.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila hard agar medium for egg collection plates. - [Read Drosophila Hard Agar Medium for Egg Collection Plates Protocol]

Drosophila Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/bloomfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila medium. - [Read Drosophila Medium Protocol]

Drosophila Semi-defined Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/germanfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila semi-defined medium. - [Read Drosophila Semi-defined Medium Protocol]

Drosophila Sucrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/neurofood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila sucrose and yeast medium. - [Read Drosophila Sucrose and Yeast Medium Protocol]

Histological Fixatives - http://www.bristol.ac.uk/vetpath/cpl/histfix.htm

Category: Histology Protocols: Histological Fixation

Fixation, Classification, REMOVAL OF FIXATION PIGMENTS, Fixative recipes, Mercury pigment, Dichromate pigment. Univ. Bristol Veterinary. - [Read Histological Fixatives]

Immunocytochemistry - http://cancer.ucsd.edu/howelllab/Immunocyto.html

Category: Immunology: Immunocytochemistry Protocols

Method for plating cells, fixing cells, antibody incubation and mounting of slides. PHEM buffer, Antifade recipes. Howell Lab. - [Read Immunocytochemistry]

In Vitro Transcription With Yeast Nuclear Extract - http://www.fhcrc.org/science/labs/hahn/methods/biochem_meth/polii_trans.html

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription with yeast nuclear extract. Includes recipes for: 5x Acetate transcription buffer; Creatine phospho kinase; Phospho creatine; Stop mix; HA + 0.1 M potassium acetate; 5x glutamate transcription buffer (5 ml). Includes also protocol for Primer Extension Assay. - [Read In Vitro Transcription With Yeast Nuclear Extract]

Lambda Phage DNA Quickprep - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/DNAIsolation/phageQuickprep.html

Category: Molecular Biology Protocols: Phage Protocols and Methods

Lambda Phage DNA Quickprep with recipes for PSB, STE, l-dil and MgCa buffers and media. Kay Schneitz - [Read Lambda Phage DNA Quickprep]

Large scale nuclear extract preparation - http://mesentsev.narod.ru/Biblioteque/Hattori.html

Category: Protein Protocols: Nuclear Extraction Protocols

Homogenize cells or tissue, Pellet nuclei, Lyse nuclei, Reprecipitate nuclear proteins, dialyze nuclear extract. Hattori M et al., DNA Cell Biol. 1990. Homogenization buffer and dialysis buffer recipes. - [Read Large scale nuclear extract preparation]

Mirmira Lab ChIP Protocol - http://faculty.virginia.edu/mirmira/resources_files/Protocols/MirmiraLabChIPprotocol.htm

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Mirmira Lab ChIP Protocol. 50-step protocol forformaldehyde crosslinking to ChIP and also provides recipes for all buffer reagents. - [Read Mirmira Lab ChIP Protocol]

Nuclear and Splicing Extract Preparation - http://penguin.uchc.edu/~intron/Protocols/NuclearS100%20Extract.html

Category: Protein Protocols: Nuclear Extraction Protocols

S100 extract cytosolic extraction preparation. Buffer A (Hypotonic Buffer), Low and High salt buffer recipes. Brent Graveley - [Read Nuclear and Splicing Extract Preparation]

Phosphoamino acid analysis - http://pingu.salk.edu/~sefton/Hyper_protocols/Paa.html

Category: Protein Protocols: Protein Phosphorylation Protocols

Recipes and protocols for the analysis of phosphoamino acids (PAA). Mark Kamps's method. Bart's Cookbook. - [Read Phosphoamino acid analysis]

Articles (1-2 of 2)

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.