recessive Protocols

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

Describes an experimental cross in mice that can be used to define and map induced germ-line mutations that map to a single chromosome. The cross is a modification and extension of a conventional three-generation recessive mutagenesis screen. Includes: The Mutagenesis Breeding Plan; Consomic Strains; Generating Mutations; Generating and Genotyping G2 Females; Genotyping G3 Progeny; Phenotyping G4 Progeny; etc.. - [Read A Targeted Screen to Detect Recessive Mutations that have Quantitative Effects Protocol]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to determine recessive-lethal mutation rates. - [Read How to Determine Recessive-Lethal Mutation Rates]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use helper strains for maintaining and crossing handicapped recessive mutants, for forcing and resolving heterokaryons, and for determining heterokaryon compatibility. - [Read How to Use Helper Strains for Maintaining and Crossing Handicapped Recessive Mutants]