real Protocols

Category: Real Time PCR

Affymetrix Real Time PCR information and optimization. Yale U. Keck Foundation. - [Read Affymetrix Real Time PCR information and optimization]

Category: PCR Protocols: Real-Time PCR Protocols

Protocol describes the real-time PCR using RNA purified from laser-captured tissues Laser Capture Microdissection (LCM): Preparation and Sectioning of Frozen Tissue Blocks and Purification of RNA from Isolated Cells. - [Read cDNA Synthesis and Real-Time PCR Using RNA from Laser-Captured Cells Protocol]

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Mapping Protein/DNA Interactions by Cross-Linking Examining the Distribution of Telomeric and DNA Repair Proteins by ChrIP and Real-Time PCR - [Read Chromatin-IP (ChrIP) Protocol]

Category: Fungi Protocols: Fungi Genetics Protocols

Standard operating procedure for the determination of tissue fungal burden utilizing quantitative real time polymerase chain reaction (QPCR). This standard operating procedure will provide information on how to assess fungal tissue burden of infected animals by use of a single copy (FKS) or multicopy gene (18s RNA) to assess the number of fungal cell nuclei present. - [Read Determination of Tissue Fungal Burden Utilizing Quantitative Real Time Polymerase Chain Reaction]

Category: Real Time PCR

Genotyping Ts65Dn mice is based on doing simultaneous quantitative PCR amplification of a gene or genes in the Ts65Dn chromosome and a control gene on another chromosome (in this case Apob) and comparing the average change (delta) in threshold cycle (CT) - [Read Genotyping Mice Using Real Time]

Category: Molecular Imaging

ImageJ - Image Processing and Analysis in Java. Useful program for MacOs MacosX and PC. Analysis includes: Measure area, mean, standard deviation, min and max of selection or entire image. Measure lengths and angles. Use real world measurement units s - [Read ImageJ - Image Processing and Analysis in Java]

Category: Microscopy Protocols: In Vivo Imaging Protocols

Positron emission tomography (PET) is a established quantitative and noninvasive imaging modality. With the PET reporter gene (PRG)/PET reporter probe (PRP) system, based on a mutant form of herpes simplex virus 1 thymidine kinase (HSV1-sr39tk), the PET signal is directly proportional to the enzymatic activity of sr39TK9-14. In this protocol, we describe in detail a method for reporter gene labeling of islets and quantitative scanning using a reporter probe. - [Read In Vivo Functional Real-Time Imaging of Transplanted Islets Using Positron Emission Tomography (PET)]

Category: PCR Protocols: Real-Time PCR Protocols

In multiplex real-time PCR, different sets of primers with different labels are used to amplify separate genes from the template DNA in one tube.  This protocol uses LUX (Light Upon eXtension) primers from invitrogen.  FAM (6-carboxy-fluorescein) is used to label the gene of interest, and JOE (6-carboxy-4', 5'-dichloro-2',7'-dimethoxy-fluorescein) is used to label a housekeeping gene as an internal control to normalize between different reactions. - [Read Multiplex Real-Time PCR Protocol]

Category: Real Time PCR: Real Time PCR Information

Allelic discrimination by nick-translation PCR with fluorogenic probes. Lee et al., 1993. - [Read Original Real Time Paper. Allelic discrimination by nick-translation PCR with fluorogenic probes.]

Category: Real Time PCR

A detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Wang et al 2003. - [Read Protocol for Real-Time RT-PCR Using SYBR Green]

Category: Cell Biology and Cell Culture

Quantitative Telomerase Detection Kit. Includes: QTD Real-Time PCR Assay Procedure; Quantitative Telomerase Detection Kit Description. - [Read Quantitative Telomerase Detection Kit]

Category: Affiliates

Real Time PCR Information and protocols. - [Read Real Time PCR Info]

Category: Real Time PCR

Real Time PCR the Basics Ambion - [Read Real Time PCR the Basics Ambion]

Category: Real Time PCR

Real-Time Chemistry, Instrumentation, Real-Time PCR Quantitation, Quantitative Primer and Probe Design, Thermal Cycling Parameters, Sample Preparation, and Real-time Data Analysis. - [Read Real-Time or Kinetic PCR]

Category: Real Time PCR

Learn Quantitative PCR Methodology. Assay parameters, Melt Curves and Primer Dimers, Standard Curves, Dynamic Range, Quantification methods, Delta CT, Standard Curves. Dr.Powell. - [Read Real-Time PCR (qPCR) Basics]

Category: Real Time PCR

Real-Time PCR Guide - Informative Background. M.Tevfik Dorak. - [Read Real-Time PCR Guide - Informative Background]

Category: PCR Protocols: Real-Time PCR Protocols

Protocol uses FAM-(6-carboxy-fluorescein) or JOE-(6-carboxy-4', 5' -dichloro-2',7' -dimethoxy-fluorescein) labeled LUX (Light Upon eXtension) primers, which can quantify 100 or fewer copies of the target DNA in a background of nonspecific templates, over a broad dynamic range of less than 100-107 copies.  It uses uracil deglycosylase (UDG) to minimize the risk of carryover contamination, and includes a melting curve analysis of the product. - [Read Real-Time PCR Protocol]

Category: PCR Protocols: Real-Time PCR Protocols

Protocol uses the Superscript II First-Strand Synthesis system for the generation of cDNA from total RNA.  RNA purified using TRIzol reagent (Invitrogen) or the methods described in Preparation of RNA Using Guanidinium Isothiocyanate/Cesium Chloride Ultracentrifugation, Preparation of RNA from Paraffin-Embedded Fixed Tissue. - [Read Real-Time RT-PCR: cDNA Synthesis Protocol]

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

Protocol uses the Superscript II First-Strand Synthesis system for the generation of cDNA from total RNA.  RNA purified using TRIzol reagent (Invitrogen) or the methods described in Preparation of RNA Using Guanidinium Isothiocyanate/Cesium Chloride Ultracentrifugation, Preparation of RNA from Paraffin-Embedded Fixed Tissue. - [Read Real-Time RT-PCR: cDNA Synthesis Protocol]

Category: Real Time PCR

Real-time/SNP ABI 7900HT Protocol PDF. :Real Time PCR - [Read Real-time/SNP ABI 7900HT Protocol PDF. :Real Time PCR]

Category: Cell Culture Protocols

Protocol describes static culture of postimplantation embryos, an alternative to the roller method. The static method is best suited to 6.0 to 7.0 days post coitum (dpc) embryos followed for 24 hours (7.0 dpc embryos) to 48 hours (6.0 dpc embryos) of development. It allows repetitive real-time observation with minimal handling of the embryo. It is especially useful if single or small groups of embryos need to be distinguished from each other. - [Read Static Culture of Postimplantation Embryos Protocol]

Category: PCR Protocols: Real-Time PCR Protocols

Protocol for suppression of insulin target gene expression by SecinH3 quantified by real-time PCR. - [Read Suppression of Insulin Target Gene Expression by SecinH3 Quantified by Real-Time PCR Protocol]

Category: Real Time PCR: Real Time PCR Information

The Usefulness of Real-time PCR in Immunogenetics. Principles, technique, applications of Real time pcr. Provenzano et al., Scientific 2001 Communications. - [Read The Usefulness of Real-time PCR in Immunogenetics]

Category: Real Time PCR

Two Step real time PCR protocol. Frank. - [Read Two Step real time PCR protocol]

Category: Real Time PCR

Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT. Dr. Craig Cadwell - [Read Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT]