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Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4603

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Using confocal laser-scanning microscope & GFP fusion proteins in time-lapse imaging to visualize the behavior of organelles and to track membrane-bound transport intermediates that bud off from organelles. Practical issues related to construction & expression of GFP fusion proteins are discussed. Essential for optimizing the brightness and expression levels of GFP fusion proteins so that intracellular membrane-bound structures containing these fusion proteins can be readily visualized. - [Read Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells]

Membrane Trafficking and Organelle Reagents - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip23

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Several common drugs, their targets, and protocols are described for studying organelle distribution and trafficking. The drugs are readily available from general suppliers, including Sigma, Roche, and Calbiochem. - [Read Membrane Trafficking and Organelle Reagents]

Purification of Fusion Proteins by Affinity Chromatography on Glutathione Agarose Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3655

Category: Chromatography Protocols: Affinity Chromatography Protocols

Recombinant proteins, constructed in pGEX vectors, are fused to glutathione S-transferase (GST) and can be purified to near homogeneity by affinity chromatography on glutathione-agarose. Bound GST-fusion proteins are readily displaced from the column by elution with buffers containing free glutathione. - [Read Purification of Fusion Proteins by Affinity Chromatography on Glutathione Agarose Protocol]

Purification of Maltose-binding Fusion Proteins by Affinity Chromatography on Amylose Resin Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4087

Category: Chromatography Protocols: Affinity Chromatography Protocols

Foreign proteins fused to maltose-binding protein can be readily purified to near homogeneity by affinity chromatography on resins containing cross-linked polysaccharides such as amylose. - [Read Purification of Maltose-binding Fusion Proteins by Affinity Chromatography on Amylose Resin Protocol]