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An Assay for RNA-Dependent RNA Polymerase Activity Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4322

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol provides a quick way to assay for RNA-dependent RNA polymerase (RdRP) activity in crude protein samples. RdRP transcription products remain at the origin during paper chromatography. - [Read An Assay for RNA-Dependent RNA Polymerase Activity Protocol]

Protocol for siRNA-Primed RNA Synthesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4326

Category: RNAi Technology Protocols

Short interfering RNAs (siRNAs) can be used to prime RNA synthesis by the RNA-dependent RNA polymerase (RdRP). SiRNAs can be used by RdRP as primers for specific cellular mRNAs, forming dsRNA products capable of inducing transitive RNAi. - [Read Protocol for siRNA-Primed RNA Synthesis Protocol]