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Cell Viability Assays that Measure Metabolic Capacity Protocol - http://www.promega.com/paguide/chap4.htm#title3

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Blue® Cell Viability Assay uses an optimized reagent containing resazurin. The homogeneous procedure involves adding the reagent directly to cells in culture at a recommended ratio of 20µl of reagent to 100µl of culture medium. - [Read Cell Viability Assays that Measure Metabolic Capacity Protocol]

DNA Cloning - http://www.molecularstation.com/dna-cloning/

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA Cloning. Cloning Calculator for ratio insert to vector / plasmid. Molecular Station. - [Read DNA Cloning]

Immunoprecipitation: Preclearing the Lysate Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4535

Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols

To reduce backgrounds and to improve the signal-to-noise ratio, an antibody that does not recognize the antigen being studied can be added to the lysate and processed as for a normal immunoprecipitation. Any nonspecific proteins that might contaminate the final immunoprecipitation step are presumably removed with this irrelevant antibody. - [Read Immunoprecipitation: Preclearing the Lysate Protocol]

Live-Cell Imaging of GFP in Plants - http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.ip31

Category: Microscopy Protocols: Live-Cell Imaging Protocols

GFP serves as a molecular marker that can be imaged dynamically in living cells, both in its native form & as a fusion to other proteins. For GFP imaging, plants present the challenge of autofluorescence from chlorophyll, lignified cell walls, vacuolar contents, and other cell materials, all of which can obscure the GFP signal. Maximizing the signal-to-noise ratio is a major concern, and careful consideration should be given to the choice of tissue imaged, GFP expression level, etc. - [Read Live-Cell Imaging of GFP in Plants]

Silver Staining Protocol - http://www.lamondlab.com/pdf/silver_staining.pdf

Category: Proteomic Protocols: Silver Staining Protocols

Silver Staining Protocol. Compatible with mass spectrometry, excellent signal-to-noise ratio. Lammond Lab. - [Read Silver Staining Protocol]

Watching Molecular Motors at Work by Video-Enhanced Light Microscopy - http://www.mih.unibas.ch/Booklet/Booklet96/Chapter2/Chapter2.html

Category: Microscopy Protocols: Optical Microscopy Protocols

The light microscope allows dynamic biological processes to be imaged in their native (i.e., aqueous) environment with relatively high temporal resolution. However, the diffraction-limited resolution is low. When working at or beyond the diffraction-limited resolution of the LM, a disadvantage of fluorescence imaging is the relatively low signal-to-noise (S/N) ratio of the images. However, this can be increased significantly by video and computer technology. - [Read Watching Molecular Motors at Work by Video-Enhanced Light Microscopy]

Articles (1-10 of 23)

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

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