rabbit Protocols

Search results for: rabbit

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Affinity Antibody Purification of Protein A/G Chromatography - http://www.upstate.com/misc/protocol_detail.q.prot.e.antibody_purification.a.name.e.Antibody_Purification

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Antibody Purification (Antiserum or Ascites by Protein A/G Chromatography). Species and Type of Antibody Agarose Rabbit IgG Protein A or Protein G Mouse IgG1 Protein G Mouse IgG2 Protein A or Protein G Mouse IgG3 Protein G Sheep IgG Protein G but binds weakly Rat IgG Protein G but binds weakly Guinea Pig IgG Protein A Dog IgG Protein A Goat IgG Protein G Pig IgG Protein A Hamster IgG Protein G. By Millipore. - [Read Affinity Antibody Purification of Protein A/G Chromatography]

Agarose Overlay Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/31.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Protocol descibes the use of L929 mouse fibroblast cells cultured in vitro in an agarose overlay assay to assess the toxicity of test substances. The assay may be useful in assessing the irritation potential of test substances (e.g. surfactant-based products) as an alternative to the Draize rabbit eye test. - [Read Agarose Overlay Assay Protocol]

Biomethodology of the Rabbit - http://research.uiowa.edu/animal/?get=rabbit

Category: Animal Research Techniques

Rabbit Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rabbit]

Polyclonal Antibody Production Protocol - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/PAb.html

Category: Antibody Protocols: Antibody Production Protocols

Protocol for polyclonal antibody production. Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Includes: Rabbit Immunization; Initial Preparation; Pre-bleed; Antigen Injection; Monitoring of Titer; Purification of Antibodies. - [Read Polyclonal Antibody Production Protocol]

Preparation of Messenger-Dependent Rabbit Reticulocyte Lysate - http://www.bio.com/protocolstools/protocol.jhtml?id=p9003

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for preparation of messenger-dependent rabbit reticulocyte lysate. Informamtion also added to test that the lysate is working before doing the Nuclease inactivation - [Read Preparation of Messenger-Dependent Rabbit Reticulocyte Lysate]

Preparation of Rabbit Reticulocyte Lysate Cell-Free System Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p308

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for the preparation of rabbit reticulocyte lysate cell-free system. Protocol includes information on: Solutions; BioReagents and Chemicals and Protcol Hints. - [Read Preparation of Rabbit Reticulocyte Lysate Cell-Free System Protocol]

Rabbit Articular Chondrocyte Functional Toxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/41.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

In this test rabbit articular chondrocytes are cultured in the presence of test compound, the toxicity of which is then determined by its effect on the production of proteoglycan by the cells, as detected by the dye Alcian Blue. - [Read Rabbit Articular Chondrocyte Functional Toxicity Test]

SIRC Cytotoxcitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/40.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Rabbit-derived corneal cells are cultured in the presence of test compounds, the toxicity of which are determined by their effect upon cell viability. A decrease in cell number, as measured by uptake of the dye Neutral Red, serves as an indicator of potential cytotoxicity. This test has been proposed as a potential replacement alternative for the Draize Eye Irritation test. - [Read SIRC Cytotoxcitiy Test]

Testing Rabbit Bleeds By Elisa - http://mitchison.med.harvard.edu/protocols/ab2.html

Category: Immunology

Coupling Peptide to BSA, ELISA with Peptide conjugated to BSA. Claire Walczak - [Read Testing Rabbit Bleeds By Elisa]

Tips and Hints for the Storage of Antibodies - http://www.sysy.com/faqs/storage.html

Category: Antibody Protocols: Antibody Production Protocols

Tips and hints for the storage of antibodies. Includes: Storage of lyophilized antibodies; Monoclonal antibodies; Rabbit antisera; Polyclonal affinity purified antibodies. - [Read Tips and Hints for the Storage of Antibodies]