Questions and answers about cell sorting. Includes: When should I use fluorescence activated cell sorting over bulk separation methods like panning or magnetic bead separations? Will my cells be harmed by the sorting process? How many cells do I need to prepare to recover 1 X 106 of a population that comprises 10% of the cells? Are there ways to improve sort recovery? etc... - [Read FAQs About Cell Sorting]
Gel Filtration Column Chromatography Protocol. Includes information on: Calibrating the column, Applying standards to the column, Running SDS-PAGE Gels (Tris-Tricine “Schägger†Gels) and includes questions. North Arizona University. - [Read Gel Filtration Column Chromatography]