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Acetone precipitation of protein from Buffer RLT or Buffer RLT - http://www1.qiagen.com/literature/protocols/pdf/RY22.pdf

Category: Protein Protocols: Protein Precipitation Procols

Acetone precipitation of protein from Buffer RLT or Buffer RLT. QIAGEN. - [Read Acetone precipitation of protein from Buffer RLT or Buffer RLT]

Detecting DNA Contamination in RT-PCR Qiagen. - http://www1.qiagen.com/resources/info/detecting_dna_contamination_in_rtpcr.aspx?

Category: RNA Protocols: RT-PCR and cDNA synthesis

Detecting DNA Contamination in RT-PCR Qiagen. - [Read Detecting DNA Contamination in RT-PCR Qiagen.]

DNA Isolation From BAC & PAC Clones Protocol - http://bacpac.chori.org/bacpacmini.htm

Category: BACs Bacterial Artificial Chromosome Protocols

Rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary. - [Read DNA Isolation From BAC & PAC Clones Protocol]

DNA Isolation From BAC & PAC Clones Protocol - http://bacpac.chori.org/bacpacmini.htm

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary. - [Read DNA Isolation From BAC & PAC Clones Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes Protocol - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for the isolation of genomic and viral DNA from lymphocytes. Includes: Viral DNA in cytosolic fraction and Viral DNA in nuclear fraction integrated. - [Read Isolation of Genomic and Viral DNA from Lymphocytes Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for the isolation of genomic and viral DNA from lymphocytes using the QIAGEN® Genomic-tip. - [Read Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip]

Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html

Category: DNA Protocols: TOPO Cloning

Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.