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Storage of Purified Antibodies - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4282

Category: Antibody Protocols: Antibody Handling Protocols

This protocol describes the storage of "homemade" purified antibodies. Antibodies from commercial sources are normally supplied with proper storage conditions. - [Read Storage of Purified Antibodies]

Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues. - http://www.axis-shield.com/densityhome/optiprep/M17.pdf

Category: Cell Biology and Cell Culture

Summary of the techniques used for the purification of viruses using pre-formed iodixanol gradients. Viruses purified include: Semliki Forest, Retrovirus (from human melanoma cells), Rabies, Polyoma, Papillomavirus, Lassa, Influenza, Bovine viral diarrhea. - [Read Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues.]

Tips and Hints for the Storage of Antibodies - http://www.sysy.com/faqs/storage.html

Category: Antibody Protocols: Antibody Production Protocols

Tips and hints for the storage of antibodies. Includes: Storage of lyophilized antibodies; Monoclonal antibodies; Rabbit antisera; Polyclonal affinity purified antibodies. - [Read Tips and Hints for the Storage of Antibodies]

Transcript Profiling by Microarray Analysis Protocol. - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000019.pdf?pid=PP00000019

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited cDNAs. The procedure requires a minimum of 5 mg of purified total RNA as starting material. Includes: First Strand cDNA Synthesis; Second Strand cDNA Synthesis; cDNA Cleanup and Precipitation; In vitro Transcription; Cleanup and Quantification of in vitro Transcribed RNA; Fluorescent Labeling of the Target Samples. - [Read Transcript Profiling by Microarray Analysis Protocol.]

Transcript Profiling by Microarray Analysis—Agilent Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000019.pdf?pid=PP00000019

Category: Microarray Protocols: Microarray DNA Labeling Protocols

Understanding Protein Trafficking in Plant Cells Through Proteomics - http://www.cepceb.ucr.edu/resources/pdf/pan/ERP-review2_781_2005.pdf

Category: Protein Protocols: Protein Trafficking

Background and methods to study plant transport. Includes new methods to study protein trafficking in plant cells, includes: Identification of protein sorting pathways in non purified samples; Localization of organelle proteins by isotype tagging/isotype-coded affinity tag; Coupling of chemical genomics and proteomics; Top down mass spectrometry; Compartment-specific markers to aid in the purification of organelles. - [Read Understanding Protein Trafficking in Plant Cells Through Proteomics]

UV Absorbance 280 nm Protein Determination - http://wolfson.huji.ac.il/purification/Protocols/OD280.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

UV Absorbance 280 nm Protein Determination. Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates or partial purified material. Quantitation of the amount of protein in a solution is possible in a simple spectrometer. Absorption of radiation in the near UV by proteins depends on the Tyr and Trp content (and to a very small extent on the amount of Phe and disulfide bonds). Dr. Mario Lebendiker - [Read UV Absorbance 280 nm Protein Determination]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

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