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Enzyme Purification by Acetone Precipitation - http://www.glue.umd.edu/~nsw/ench485/lab6b.htm

Category: Protein Protocols: Protein Precipitation Procols

Acetone Precipitation Protocol and Method. Nam Sun Wang. Univ. Maryland. - [Read Enzyme Purification by Acetone Precipitation]

Enzyme Purification by Salt Ammonium Sulfate Precipitation - http://www.ece.umd.edu/~nsw/ench485/lab6a.htm

Category: Protein Protocols: Protein Precipitation Procols

ENZYME PURIFICATION BY SALT (AMMONIUM SULFATE) PRECIPITATION. Prepared by Nam Sun Wang, Department of Chemical & Biomolecular Engineering. University of Maryland. - [Read Enzyme Purification by Salt Ammonium Sulfate Precipitation]

Epitope Tagging of Recombinant Proteins Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E663998FCCB61070F32F1EAE0301F7F&objectid=6677C749FA75FD435618AC07256968A7

Category: Immunology: Immunoprecipitation Protocols

Protocols for detection and purification of proteins tagged with a particular epitope, the FLAG tag, although the same general approach can be applied to other epitope tags. The protocols employ the anti-FLAG M2 antibody to detect and purify FLAG-tagged proteins. The methods presented are immunoprecipitation of FLAG fusion proteins from cells using an anti-FLAG M2 affinity gel, detection of FLAG fusion proteins by western blotting, and purification of FLAG fusion proteins by anti-FLAG. - [Read Epitope Tagging of Recombinant Proteins Protocol]

Expression and Purification of His Tagged Proteins in E. coli Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/H1.html

Category: E Coli Protocols: E coli Protein Protocols

Protocol for the expression and purification of His tagged proteins in E. coli. - [Read Expression and Purification of His Tagged Proteins in E. coli Protocol]

Expression of a prokaryotic P-type ATPase in E. coli Plasma Membranes and Purification by Ni2+-affin - http://www.biologicalprocedures.com/bpo/arts/1/9/m9.htm

Category: Recombinant Protein Protocols

Expression of a prokaryotic P-type ATPase in E. coli Plasma Membranes and Purification by Ni2+-affinity chromatography. Biological Procedures Online - [Read Expression of a prokaryotic P-type ATPase in E. coli Plasma Membranes and Purification by Ni2+-affin]

Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4125

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from blood samples. - [Read Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display]

Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4124

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from tissue samples. - [Read Extraction and Purification of RNA from Tissue Samples for Fluorescent mRNA Differential Display]

Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4123

Category: PCR Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from the tissue-cultured cells of interest. For other starting materials, such as blood samples, please see Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display. - [Read Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential]

Extraction and Purification of Total RNA using TRIzol OR TRI Reagent Protocol - http://www.animal.ufl.edu/hansen/Protocols/RNA_extraction.htm

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols

Protocol for extraction and purification of total RNA using TRIzol OR TRI reagent. Includes: Homogenization for Cell Suspensions; Phase Separation; RNA Precipitation; RNA Wash; Redissolving the RNA; Determination of RNA Concentration and Purity; Preparation of Rnase-free water. - [Read Extraction and Purification of Total RNA using TRIzol OR TRI Reagent Protocol]

Fan Lab Bisulfite Treatment of Genomic DNA - http://www.genetics.ucla.edu/labs/fan/Protocols_Bisulfite.htm

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

A detailed protocol for bisulfite treatment of DNA. DNA is first digested with a restriction enzyme. DNA is denauted at 97C for 5min. Bisulfite protocol is included. Also DNA purification using the Promega Wizard DNA Cleanup kit in indicated. Fan Lab. UCL - [Read Fan Lab Bisulfite Treatment of Genomic DNA]

Fractionation of T and B Cells Using Magnetic Beads Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E66367B022BC1A53B67DF9C702B2513&objectid=66749A4CD26365F08DFBDE734F6A50F5

Category: Immunology: Mononuclear Cell Isolation Protocols: B Lymphocyte Isolation Protocols

Protocol describes the purification of mouse T cells, B cells, and T cell subsets using magnetic bead separation. Isolation of cell subsets using magnetic beads is quick, simple, and reliable and can result in high yields of very pure cells. - [Read Fractionation of T and B Cells Using Magnetic Beads Protocol]

Category: Immunology: Mononuclear Cell Isolation Protocols: T-Cell Isolation Protocols

Fragment purification on Sephacryl S-500 spin columns - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.H

Category: Chromatography Protocols: Column Chromatography Protocols

Fragment purification on Sephacryl S-500 spin columns , Bruce A. Roe. - [Read Fragment purification on Sephacryl S-500 spin columns]

Glutamate Oxaloacetic Acid Transaminase (GOT) Purification Precipitation - http://www.bio.mtu.edu/campbell/bl4820/lectures/lec5/482lec5.pdf

Category: Protein Protocols: Protein Precipitation Procols

Glutamate Oxaloacetic Acid Transaminase (GOT) Purification Precipitation. Wilbur H. Campbell. - [Read Glutamate Oxaloacetic Acid Transaminase (GOT) Purification Precipitation]

Gradient Purification of AT2 Inactivated HIV-1 Protocol - http://ubik.microbiol.washington.edu/protocols/bl3/at2.htm

Category: Viral Protocols

Protocol for gradient purification of AT2 inactivated HIV-1. - [Read Gradient Purification of AT2 Inactivated HIV-1 Protocol]

Growth and Purification of 25-100 ug Lambda Clone DNA Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda13.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

There are essentially three parts to this protocol: 1. growth of at least 5x10e8 pfu phage to provide an inoculum growth of a larger liquid lysate that will produce about 5x10e12 pfu; 2. concentration and purification of the phage, and; 3. DNA preparation. - [Read Growth and Purification of 25-100 ug Lambda Clone DNA Protocol]

GST Fusion Protein Prep - http://cmgm.stanford.edu/~vesicle/protocols/gst_prot.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Detailed protocols and methods for GST protein purification. Vesicle Trafficking, Stanford. - [Read GST Fusion Protein Prep]

GST Fusion Protein Purification from Yeast - http://www.med.unc.edu/~hdohlman/gst.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

GST Fusion Protein Purification from Yeast. Lysis Buffer recipe for yeast, glutathione bead method for GST purification. Dohlman Lab - [Read GST Fusion Protein Purification from Yeast]

GST Fusion Protein Purification from Yeast Protocol - http://www.med.unc.edu/~hdohlman/gst.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Protocol for GST fusion protein purification from Yeast. - [Read GST Fusion Protein Purification from Yeast Protocol]

Home-made Taq Polymerase Purification - http://www.nottingham.ac.uk/~mbzspd/methods/Home_made_Taq_polyme.html

Category: PCR Protocols

PCR polymerase costs can be high. If you are willing to work, you can produce bacteria containing the clone. It appears to produce lots of Taq and is quite stable. The proceedure takes 4 days start to (15 000 units of Taq) finish. The Taq also appears ver - [Read Home-made Taq Polymerase Purification]

Immunoaffinity Purification Gibberellins - PDF - http://www.plantphysiol.org/cgi/reprint/90/2/445.pdf

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Immunoaffinity Purification Gibberellins. PDF. - [Read Immunoaffinity Purification Gibberellins - PDF]

Immunoaffinity Purification: Coupling Antibodies to Protein A or G Bead Columns Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4303

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Protocol describes how antibodies are bound to protein A or protein G beads and are then crosslinked to the matrix via a bifunctional coupling reagent called dimethyl pimelimidate (DMP). - [Read Immunoaffinity Purification: Coupling Antibodies to Protein A or G Bead Columns Protocol]

Immunoaffinity Purification: Eluting Antigens from Immunoaffinity Columns Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4276

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

This elution procedure relies on disrupting the bonds between the antibody and the antigen. - [Read Immunoaffinity Purification: Eluting Antigens from Immunoaffinity Columns Protocol]

In Vitro Footprinting Methods Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/III.%20Proteins/C.%20DNA-Protein%20Interactions/5.%20In-vitro%20footprinting.pdf

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Protocol for in vitro footprinting methods. Includes: Preparation of labeled probes; Gel purification. - [Read In Vitro Footprinting Methods Protocol]

Isolation and Purification of Tetrahymena Nuclei Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4500

Category: Tetrahymena Protozoa Protocols

Protocol describes how to allow the isolation of nuclei from all stages of the Tetrahymena life cycle in high yield with a high degree of purity. This method gives highly purified populations of both micronuclei and macronuclei. - [Read Isolation and Purification of Tetrahymena Nuclei Protocol]

Articles (1-4 of 4)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

DNA Fragment Purification from Polyacrylamide Gels Protocol

A simple protocol for the purification of DNA fragments from Polyacrylamide Gels.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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