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Advanced Methodologies in Flow Cytometry - http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/index1.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Advanced Methodologies in Flow Cytometry. Andrea Cossarizza et al.. Materials from a workshop held March, 1997 in Modena, Italy. - [Read Advanced Methodologies in Flow Cytometry]

Analysis of DNA Content and Surface Immunophenotype Protocol Using Ethanol Fixation - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/ethanol.htm

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. - [Read Analysis of DNA Content and Surface Immunophenotype Protocol Using Ethanol Fixation]

Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1 - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/cossar1/cossariz.htm

Category: Cell Organelle Protocols: Mitochondria Protocols

The technique of JC-1 staining has been developed with the intent to detect DY in intact, viable cells. For this purpose JC-1 acts as a marker of mitochondrial activity, since the formation of J-aggregates, which give red emission, is reversible. Cells with high DY are those forming J-aggregates, thus showing high red fluorescence. On the other hand, cells with low DY are those in which JC-1 maintains (or re-acquire) monomeric form, thus showing only green fluorescence. - [Read Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1]

Annexin V Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap16.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Annexin V, belonging to a recently discovered family of proteins, the annexins, with anticoagulant properties has proven to be a useful tool in detecting apoptotic cells since it preferentially binds to negatively charged phospholipids like PS in the presence of Ca2+ and shows minimal binding to phosphatidylcholine and sphingomyeline. Changes in PS asymmetry, which is analyzed by measuring Annexin V binding to the cell membrane, were detected before morphological changes associated with... - [Read Annexin V Protocol]

Annexin V Staining Flow Cytometry Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/page1.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Annexin V Staining Flow Cytometry Protocol - a general protocol for staining cell for cytometry analysis. BD PharMingen. - [Read Annexin V Staining Flow Cytometry Protocol]

Caspase Detection Methods for Apoptosis - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap11.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Caspase Detection Methods for Apoptosis. Zeuner et al. Purdue Cytometry CD-ROM Series. - [Read Caspase Detection Methods for Apoptosis]

Detailed 2D Gel Electrophoresis - http://www.purdue.edu/dp/bbc_labs/2d.html

Category: Proteomic Protocols: 2-D Gel Electrophoresis

Excellent Detailed Protocol for 2-D Gel Electrophoresis including staining, gel running, and equipment. Purdue PSAL. - [Read Detailed 2D Gel Electrophoresis]

Detection of Apoptosis vs Necrosis (Oncosis) - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap10.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Detection of Apoptosis vs Necrosis (Oncosis). Amazing review of methods. Vitale et al. - [Read Detection of Apoptosis vs Necrosis (Oncosis)]

Detection of apoptotic process in situ using immunocytochemical - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/barbie.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Detection of apoptotic process in situ using immunocytochemical and TUNEL assays. Barbieri et al. Purdue Cytometry CD-ROM Series - [Read Detection of apoptotic process in situ using immunocytochemical]

Flow Cytometric Analysis of Gram-Negative Bacterial Cells - http://www.cyto.purdue.edu/flowcyt/research/micrflow/robert/robert.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Flow cytometric analysis of gram-negative bacterial cells. Includes: Staining Conditions and FACSort Settings; Light Scatter Resolution on the BD FACSort; Bacterial Cell Resolution on the BD FACSort. - [Read Flow Cytometric Analysis of Gram-Negative Bacterial Cells]

Flow Cytometry Laboratory - http://www.cyto.purdue.edu/cdroms/flow/vol3/8/data/icrf/apop.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Flow Cytometry Laboratory. Apoptosis. Perdue. - [Read Flow Cytometry Laboratory]

Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/cyclinip.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol for immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry. - [Read Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry Protocol]

Phenotype-Specific Immunodetection of Cyclins using 488/630 nm Dual Laser Flow Cytometry Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/cyclind.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol for phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry. This protocol is for use with the D and E cyclins and employs 488 nm argon laser excitation of propidium iodide and a FITC-conjugated phenotypic label, and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression. - [Read Phenotype-Specific Immunodetection of Cyclins using 488/630 nm Dual Laser Flow Cytometry Protocol]

PLANT CELL, TISSUE AND ORGAN CULTURE - 1998 - http://www.hort.purdue.edu/hort/courses/HORT515/Callus-Ref.html

Category: Plant Biology Protocols: Plant Tissue Culture

Dodds, JH and LW Roberts 1995 Experiments in Plant Tissue Culture. ... 19-60 In Plant Cell Culture Protocols, Humana Press, Totowa ... - [Read PLANT CELL, TISSUE AND ORGAN CULTURE - 1998]

Simultaneous Analysis of DNA Content and Surface Immunophenotype Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/ethanol.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

In an attempt to accurately measure DNA content with simultaneous preservation of cell surface markers, we have utilized gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association. For some cell types, the presence of apoptotic cells based on reduced DNA content can also be detected. One such technique employs the addition of ethanol to cells previously resuspended in high concentrations of fetal bovine serum... - [Read Simultaneous Analysis of DNA Content and Surface Immunophenotype Protocol]