pseudopregnant Protocols

Search results for: pseudopregnant

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Blastocyst Embryo Transfer into Pseudopregnant Mouse Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp6.html

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol for blastocyst embryo transfer into pseudopregnant recipient female mouse. - [Read Blastocyst Embryo Transfer into Pseudopregnant Mouse Protocol]

Mouse Embryo Transfer into Pseudopregnant Recipient Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp2.html

Category: Mouse Protocols: Mouse Embryology Protocols

1-cell embryo transfer into pseudopregnant recipient female mouse. - [Read Mouse Embryo Transfer into Pseudopregnant Recipient Protocol]

Oviduct Transfer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4379

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes oviduct transfer of mouse embryos. It is based on the Whittingham method, which applied a well described procedure for the rat to the mouse. It is best to practice this procedure first on a cadaver and then on an anesthetized 0.5-dpc pseudopregnant mouse using a dye solution or blue Affigel beads rather than embryos to gain experience in finding the opening of the oviduct (infundibulum). - [Read Oviduct Transfer Protocol]

Uterine Transfer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4380

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a procedure for uterine transfer, which is used for chimera production. The method is based on extensive work which resulted in the first successful development and birth of in-vitro-cultured mouse embryos. It is best to practice this procedure first on a cadaver and then on an anesthetized 2.5-days post coitum (dpc) pseudopregnant mouse using blue Affigel beads rather than embryos. - [Read Uterine Transfer Protocol]

Vasectomy for Generation of Sterile Males: Access via Abdominal Wall Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4376

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a method for vasectomy in which the vas deferens is accessed through the abdominal wall. Mice are ready for mating after ~10-14 days. Vasectomized males can be bred with fertile females to obtain plugs for timed matings. The pseudopregnant females can then be used for oviduct and uterine transfers. For an alternative protocol, see Vasectomy for Generation of Sterile Males: Access via Scrotal Sac. - [Read Vasectomy for Generation of Sterile Males: Access via Abdominal Wall Protocol]

Vasectomy for Generation of Sterile Mouse Males: Access via Scrotal Sac Protocols - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4377

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a method for vasectomy in which the vas deferens is accessed through the scrotal sac. Mice are ready for mating after ~10-14 days. Vasectomized males can be bred with fertile females to obtain plugs for timed matings. The pseudopregnant females can then be used for oviduct and uterine transfers. - [Read Vasectomy for Generation of Sterile Mouse Males: Access via Scrotal Sac Protocols]