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Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Search Results Protocol Links Sort by: Hits | Alphabetical

Protein Precipitation Protocols - http://www.ls.huji.ac.il/~purification/Protocols/ProteinPrecipitation.html

Category: Protein Protocols: Protein Precipitation Procols

TCA-DOC Normal, TCA Acetone Precipitation, Ethanol Precipitation, TCA-DOC / Acetone, Acidified Acetone/Methanol, Dr. Mario Lebendiker. University of Jerusalem. - [Read Protein Precipitation Protocols]

Isolation of Protein from Tissue - http://www.ohsu.edu/nsi/faculty/reddyh/lab/protprot.html

Category: Protein Protocols: Protein Extraction From Tissue

Isolation of Protein from Tissue. P. H. Reddy, Ph.D. - [Read Isolation of Protein from Tissue]

TCA protein precipitation protocol PDF - http://www.its.caltech.edu/~bjorker/TCA_ppt_protocol.pdf

Category: Protein Protocols: Protein Precipitation Procols

Simple TCA method with recipe. TCA protein precipitation protocol. Luis Sanchez Caltech. - [Read TCA protein precipitation protocol PDF]

Co-immunoprecipitation Analysis of Protein-Protein Interactions - http://www.uphs.upenn.edu/mcrc/morrisey/coip.htm

Category: Protein Protocols: Co-immunoprecipitation

Co-immunoprecipitation Analysis of Protein-Protein Interactions. Morrisey Lab - [Read Co-immunoprecipitation Analysis of Protein-Protein Interactions]

Protein Dialysis Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p9054

Category: Protein Protocols: Protein Dialysis Protocols

Protein Dialysis Protocol. Bioprotocol. Preparation of Dialysis Tubing, Use of the Dialysis Tubing and Dialysis buffer. - [Read Protein Dialysis Protocol]

Analysis of Proteins by Immunoprecipitation - http://dps.ufl.edu/hansen/protocols/imp98.prt.htm

Category: Protein Protocols: Immunoprecipitation Protocols

Antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody. Immunoprecipitation protocols / methodology and technical background information. P.J. Ha - [Read Analysis of Proteins by Immunoprecipitation]

Transient transfection into 293T cells - http://www.cbrinstitute.org/labs/springer/protocols/jun_293T.html

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Transient transfection into 293T cells Protocol. transfection, calcium phosphate, overexpression, protein production. Jun Takagi - [Read Transient transfection into 293T cells]

Cell Lysate Extracts - http://www.upstate.com/misc/protocol_detail.q.prot.e.CellLysate_Prep.a.name.e.Cell_Lysate_Extracts-General_Protocols

Category: Protein Protocols: Protein Extraction From Cells

Cell Lysate Extracts. Great protocols for cell lysis preparation from a variety of cell types. There are numerous methods of cell stimulation and lysis. For a given protein, Upstateâ€™s Laboratories determine the specific treatment upon initial testing of its products. It is important to select the correct cell line, stimulation procedure (if any), and lysis protocol. Upstate. - [Read Cell Lysate Extracts]

EMSA Protocol using 32-p. - http://www.well.ox.ac.uk/flint/EMSA.htm

Category: DNA Protocols: EMSA DNA-Protein Protocols

Primer design, Primer annealing, Primer labelling, Probe purification, In vitro hybridization DNA-protein Binding reaction. Jonathan Flint. - [Read EMSA Protocol using 32-p.]

Immunoprecipitation of Proteins Method - IP protocol - http://www.upstate.com/misc/protocols.q.prot.e.immuno/Immunoprecipitation+of+Proteins

Category: Protein Protocols: Immunoprecipitation Protocols

Ip Protocol using protein A or G beads. Upstate. - [Read Immunoprecipitation of Proteins Method - IP protocol]

Protein Precipitation - http://www.aai.org/committees/education/Curriculum/protein.htm

Category: Protein Protocols: Protein Precipitation Procols

Protein Precipitation. Developed by: Eric Burtson. American Association of Immunologists 1995. In this lab you will separate a solution of proteins using protein precipitation. Since you will be testing the same protein mix that you used in the Gel Filtration lesson, you will pass the protein through a gel filtration column to identify which protein(s) precipitates. - [Read Protein Precipitation]

Cross-linking and Immunoprecipitation Protocol - http://www.molecularstation.com/protein/protein-protocols/immunoprecipitation-protocols/

Category: Protein Protocols: Immunoprecipitation Protocols

Cross-linking and Immunoprecipitation Protocol. Molecular Station. - [Read Cross-linking and Immunoprecipitation Protocol]

Protein Purification: Column Chromatography - Ion exchange; Dialysis and Concentration - http://wine1.sb.fsu.edu/bch5425/lect30/lect30.htm

Category: Protein Protocols: Protein Purification and Identification

Protein Purification: Column Chromatography - Ion exchange; Dialysis and Concentration. Blaber lecture - [Read Protein Purification: Column Chromatography - Ion exchange; Dialysis and Concentration]

Gel Mobility Shift Assay in PDF format - http://www.biochem.northwestern.edu/ibis/morimoto/Protocols/III.%20Proteins/C.%20DNA-Protein%20Interactions/3a.%20GMSA.pdf

Category: DNA Protocols: EMSA DNA-Protein Protocols

This protocol works well for HSF DNA-binding activity experiments. - [Read Gel Mobility Shift Assay in PDF format]

TCA/Acetone Precipitation (Large Scale) PDF - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000148.pdf?pid=PP00000148

Category: Protein Protocols: Protein Precipitation Procols

A protein precipitation procedure used to precipitate protein from cell lysates. Allows for optimal protein recovery and accurate assays. The Signaling Gateway - [Read TCA/Acetone Precipitation (Large Scale) PDF]

Analysis of Protein Gels (SDS-PAGE) - http://www.ruf.rice.edu/~bioslabs/studies/sds-page/gellab3.html

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Acetone precipitation of protein from Buffer RLT or Buffer RLT - http://www1.qiagen.com/literature/protocols/pdf/RY22.pdf

Category: Protein Protocols: Protein Precipitation Procols

Acetone precipitation of protein from Buffer RLT or Buffer RLT. QIAGEN. - [Read Acetone precipitation of protein from Buffer RLT or Buffer RLT]

SDS-PAGE - http://www.ciwemb.edu/labs/koshland/Protocols/Protein/sdspage.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS-PAGE: gel electrophoresis of proteins. Very simple protocol for SDS-PAGE. Koshland Lab, Carnegie Institution of Washington, Howard Hughes Medical Institute. - [Read SDS-PAGE]

Plant Protein Isolation - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/ProteinIsolation.pdf

Category: Protein Protocols: Protein Extraction From Tissue

Plant Protein Isolation - Rapid isolation of protein for SDS-PAGE analysis. (Essentially the same protocol as that described for GUS Assays). Stockinger Lab - [Read Plant Protein Isolation]

Protein extraction from whole tissues for isoelectric focusing - http://proteomics.missouri.edu/protocols/proteinExtraction.html

Category: Protein Protocols: Protein Extraction From Tissue

Protein extraction from whole tissues for isoelectric focusing. University of Missouri - Columbia Proteomics Center. SDS extraction followed by acetone precipitation. Also has Phenol extraction followed by methanolic ammonium acetate precipitation. - [Read Protein extraction from whole tissues for isoelectric focusing]

Mass spectrometry analysis and identification of proteins - http://www.healthsystem.virginia.edu/internet/biomolec/mass_spectrometry.cfm

Category: Mass Spectrometry Protocols

Protein sequencing by mass spectrometry. Mass analysis of peptides and proteins. Identification of modifications in proteins. Good information.Univ. Virginia. Dr. Nicholas Sherman - [Read Mass spectrometry analysis and identification of proteins]

Analysis of protein phosphorylation: methods and strategies PDF - http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1365-313X.2005.02613.x

Category: Protein Protocols: Protein Phosphorylation Protocols

Paper describing methods for monitoring kinase activity, investigating kinaseâ€“substrate specificity, examining phosphorylation in planta and the determination of phosphorylation sites in a protein. In addition, strategic considerations for experimental design and variables will be discussed. Scott C. Peck, the Plant Journal. - [Read Analysis of protein phosphorylation: methods and strategies PDF]

Lowry Protein Assay - http://www.animal.ufl.edu/hansen/protocols/LOWRY.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay. The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-275 (1951)]. Under alkaline conditions, copper complexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent binds to the protein. Bound reagent is slowly reduced and changes color from yellow to blue. P.J. Hansen, Dept. of Animal Sciences, University of Florida. - [Read Lowry Protein Assay]

Protein Purification: Assays, Specific Activity, Initial Fractionation - http://wine1.sb.fsu.edu/bch5425/lect29/lect29.htm

Category: Protein Protocols: Protein Purification and Identification

Protein Purification: Assays, Specific Activity, Initial Fractionation. Blaber Lecture. - [Read Protein Purification: Assays, Specific Activity, Initial Fractionation]

Protein Isolation and Analysis From Plants - http://csm.jmu.edu/biology/courses/bio480_580/mblab/protein_isolation.htm

Category: Protein Protocols: Protein Extraction From Tissue

Protein Isolation and Analysis From Plants. T. Rife. Department of Biology, James Madison University. Harrisonburg, VA - [Read Protein Isolation and Analysis From Plants]

Articles (1-10 of 10)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

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