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EMSA protocol (Word Doc) - http://www.med.umich.edu/intmed/endocrinology/hammerlab/Protocols/EMSA%20Protocol.doc

Category: DNA Protocols: EMSA DNA-Protein Protocols

Short EMSA protcol starting with gel running and supershift assay. Hammer Lab. - [Read EMSA protocol (Word Doc)]

Isolation of proteins from inclusion bodies - http://www.its.caltech.edu/%7Ebjorker/Isolation%20of%20proteins%20from%20.pdf

Category: Recombinant Protein Protocols

Isolation of proteins from inclusion bodies Protcol. Expression of recombinant proteins. Dissolving of recombinant protein. Refolding buffer. BJÃ–RKman group - [Read Isolation of proteins from inclusion bodies]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Category: Cell Organelle Protocols: Microsome Protocols

Protcol relating to microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in yeast. Includes spheroplast formation. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass]

Preparation of Rabbit Reticulocyte Lysate Cell-Free System Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p308

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for the preparation of rabbit reticulocyte lysate cell-free system. Protocol includes information on: Solutions; BioReagents and Chemicals and Protcol Hints. - [Read Preparation of Rabbit Reticulocyte Lysate Cell-Free System Protocol]

Protcol for In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast - http://www.bio.com/protocolstools/protocol.jhtml?id=p1831

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol includes information on: Preparation of Membranes; One-Stage Reaction; Two-Stage Reaction using Normal Amount of Membranes; Two-Stage Reaction using Low Concentration of Membranes. - [Read Protcol for In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast]

Timing of Cycles Protocol - http://homepages.gac.edu/~cellab/chpts/chpt11/ex11-5.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Protcol for the timing of cycles. - [Read Timing of Cycles Protocol]