promega Protocols

Category: Reporter Gene Assays: Luciferase Assay Protocols

Luciferase assay using a 24 well plate. Includes: Cell Lysis; Reagent Preparation; Luminescence measurement; Wash injector; Protein quantitation; Data Analysis. - [Read Luciferase Assay 24 Well Plate (Promega System)]

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Mitogen Activated Protein Kinase Guide. MEK, MEKK, MAPK, MAPKK, antibodies and kits Promega. - [Read Mitogen Activated Protein Kinase Guide]

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for monoamine oxidase assay. - [Read Monoamine Oxidase Assay Protocol]

Category: Reporter Gene Assays: GFP Assay Protocols

Green fluorescent protein is commonly used to monitor gene expression and protein trafficking within intact cells. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). - [Read Monster Green® Fluorescent Protein Assay]

Category: RNA Protocols: mRNA Extraction Protocols

mRNA Preparation. Promega. Abstract for PolyATtract® mRNA Isolation Systems - [Read mRNA Preparation]

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The latest generation of Promega cell-based assays includes luminescent and fluorescent chemistries to measure markers of cell viability, cytotoxicity and apoptosis, as well as to perform reporter analysis. Using these tools researchers can investigate how cells respond to growth factors, cytokines, hormones, mitogens, radiation, effectors, compound libraries and other signaling molecules. The protocols provided are guidelines for multiplexing cell-based assays & are intended as starting points. - [Read Multiplexing Cell Viability Assays Protocols]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol describes a system which includes all of the necessary components for in vitro transcription as well as a positive control template that provides run-off transcripts from a CMV immediate early promoter. This system is designed for runoff transcription. Alternatively, transcription products can be analyzed by primer extension. - [Read Nuclear Extract in vitro Transcription System]

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for P450 screening system. - [Read P450 Screening Systems Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Guide - Promega - [Read PCR Guide - Promega]

Category: PCR Protocols

PCR Guide - Promega - [Read PCR Guide - Promega]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Promega PCR Guide - [Read Promega PCR Guide]

Category: PCR Protocols

Promega PCR Guide - [Read Promega PCR Guide]

Category: Protein Protocols: Protein Expression Protocols

Protein Expression Protocols and Applications Guide PDF. Promega. In vitro translation systems in Prokaryotes and Eukaryotes. Promega. - [Read Protein Expression Protocols and Applications Guide PDF]

Category: RNA Protocols: In Vitro Transcription T7 SP6 T3 Protocols

Ribomax T7 In Vitro Transcription Protocol. Synthesis of mg quantities of RNA from a T7 promoter. Promega. - [Read Ribomax T7 In Vitro Transcription Protocol]

Category: RNA Protocols: In Vitro Transcription T7 SP6 T3 Protocols

Riboprobe® in vitro Transcription Systems Promega. For the in vitro synthesis of RNA. For large quantities of RNA, use Ribomax very good kit. - [Read Riboprobe in vitro Transcription Protocol]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

In vitro transcription systems includes instructions for use of products P1420, P1430, P1440, P1450 and P1460.Includes information and protocols on RNA Transcription in vitro. Information on DNA Template Preparation;Synthesis of High-Specific-Activity Radio labeled RNA Probes;Determining Percent Incorporation and Probe Specific Activity;Removal of the DNA Template Following Transcription;Removal of unincorporated nucleotides;Synthesis of large amounts of RNA;Capping RNA for in vitro translation. - [Read Riboprobe In Vitro Transcription Systems]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Information for protocol using single-tube, coupled transcription/translation reactions for eukaryotic in vitro translation. Includes information on: Translation Procedure; Positive Control Translation Reactions Using Luciferase; Cotranslational Processing Using Canine Pancreatic Microsomal Membranes; Post-Translational Analysis; Positive Control Luciferase Assays; Composition of Buffers and Solutions; Luciferase SP6/T7 Control DNAs - [Read Single-tube Coupled Transcription/Translation Reactions for Eukaryotic In Vitro]

Category: Genetics and Genomics: Genotyping Protocols

SNP Genotyping System. Includes: Protocol for Manual READIT® Assay in Multiwell Plates; Preparation of L/L Reagent; Preparation of Heating Block; CIAP/Exo Treatment; Preparation of Interrogation Probe Solutions; Template Denaturation; Sample Interrogation; Protocol for Manual Plate Reading Luminometers; Protocol for Injecting Plate Reading Luminometers; Protocol for Automated READIT® Assay with the Biomek® 2000 Instrument. - [Read SNP Genotyping System]

Category: DNA Protocols: T4 Polynucleotide Kinase Protocols

T4 Polynucleotide Kinase Protocol Promega. 5´ End-Labeling Protocol, Non-Radioactive Kinase Reaction, Exchange Reaction Protocol Using Protruding 5´ Phosphoryl Termini as Templates. Promega. T4 PNK Protocols. - [Read T4 Polynucleotide Kinase Protocol Promega]

Category: RNA Protocols: cDNA Libraries Library

Universal RiboClone® cDNA Synthesis System Protocol. Method from Promega - [Read Universal RiboClone® cDNA Synthesis System Promega]