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3-7 Assay Protocol - http://www.promega.com/tbs/tb323/tb323.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for 3/7 assay. Includes: Detection of Caspase-3 and -7 Activities in Cell-Based Assays; Detection of Caspase-3 or -7 Activity Using Purified Caspases. - [Read 3-7 Assay Protocol]

A practical guide to DNA methylation Promega PDF - http://www.promega.com/pnotes/42/methv3/Methv3_core.pdf

Category: Epigenetics and Methylation Protocols

The pattern and extent of DNA methylation can significantly affect the success of restriction digestions or bacterial transformations. Prokaryotic DNA may be methylated by host restriction/modification systems, while eukaryotic DNA often is methylated i - [Read A practical guide to DNA methylation Promega PDF]

Apoptosis Info and Protocols Promega - http://www.promega.com/paguide/chap3.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Apoptosis Info and Protocols a Promega Guide. - [Read Apoptosis Info and Protocols Promega]

Assays to Detect Apoptosis Protocol - http://www.promega.com/paguide/chap4.htm#title5

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol and methods on assays to detect apoptosis. - [Read Assays to Detect Apoptosis Protocol]

Automated CellTiter-Blue Cell Viability Assay Protocol - http://www.promega.com/tbs/ep015/ep015.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol on automated cell titer- blue cell viability assay. - [Read Automated CellTiter-Blue Cell Viability Assay Protocol]

Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/ep014/ep014.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for the automated cell titer- glo luminescent cell viability assay. - [Read Automated CellTiter-Glo Luminescent Cell Viability Assay Protocol]

Caspase 8 Assay Protocol - http://www.promega.com/tbs/tb332/tb332.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 8 assay. Includes: Detection of Caspase-8 Activity Using Purified Caspases; Detection of Caspase-8 Activity in Cell-Based Assays. - [Read Caspase 8 Assay Protocol]

Caspase 9 Assay Protocol - http://www.promega.com/tbs/tb333/tb333.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 9 assay. Includes: Detection of Caspase-9 Activity Using Purified Caspases; Detection of Caspase-9 Activity in Cell-Based Assays. - [Read Caspase 9 Assay Protocol]

Cell Viability Assays that Measure ATP Protocol - http://www.promega.com/paguide/chap4.htm#title2

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method to determine the number of viable cells in culture. Detection is based on using the luciferase reaction to measure the amount of ATP from viable cells. The amount of ATP in cells correlates with cell viability. - [Read Cell Viability Assays that Measure ATP Protocol]

Cell Viability Assays that Measure Metabolic Capacity Protocol - http://www.promega.com/paguide/chap4.htm#title3

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Blue® Cell Viability Assay uses an optimized reagent containing resazurin. The homogeneous procedure involves adding the reagent directly to cells in culture at a recommended ratio of 20µl of reagent to 100µl of culture medium. - [Read Cell Viability Assays that Measure Metabolic Capacity Protocol]

Cell Viability Information For Protocols and Applications - http://www.promega.com/paguide/chap4.htm

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Choosing a cell viability or cytotoxicity assay from among the many different options available can be a challenging task. Includes information on: Establishing an In Vitro Model System; Choosing an Endpoint to Measure; Characterizing Assay Responsiveness; Determining Dose and Duration of Exposure; Homogeneous Assays for Multiwell Formats and Automated Screening; Additional Factors to Consider When Choosing a Cell Viability Assay; Cell Viability Assays that Measure ATP Protocol; etc.. - [Read Cell Viability Information For Protocols and Applications]

CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/tb288/tb288.pdf

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for cell titer- glo luminescent cell viability assay. - [Read CellTiter-Glo Luminescent Cell Viability Assay Protocol]

CellTiter-Glo Luminescent Cell Viability Assay Protocol - http://www.promega.com/tbs/tb288/tb288.pdf

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Protocol for CellTiter-Glo luminescent cell viability assay. This assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. - [Read CellTiter-Glo Luminescent Cell Viability Assay Protocol]

Cloning Enzymes a Guide Promega - http://www.promega.com/guides/cloning_guide/default.htm

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Cloning Enzymes a Guide Promega. An Enzyme Resource Guide series, highlights those enzymes important in nucleic acid cloning procedures. Promega - [Read Cloning Enzymes a Guide Promega]

Cytochrome P450 Assay Protocol - http://www.promega.com/tbs/tb325/tb325.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for cytochrome P450 assay. - [Read Cytochrome P450 Assay Protocol]

Cytochrome P450 CYP450 Assay Protocol - http://www.promega.com/tbs/ep026/ep026.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for cytochrome P450 CYP450 assay. - [Read Cytochrome P450 CYP450 Assay Protocol]

CytoTox-ONE Homogeneous Membrane Integrity Assay Protocol - http://www.promega.com/tbs/ep016/ep016.pdf

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Protocol describes automation of the CytoTox-ONE Homogeneous Membrane Integrity Assay. - [Read CytoTox-ONE Homogeneous Membrane Integrity Assay Protocol]

Determining Number of Live and Dead Cells in Cell Population: Cytotoxicity Assay Protocol - http://www.promega.com/paguide/chap4.htm#title4

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Cell-based assays are important tools for contemporary biology and drug discovery because of their predictive potential for in vivo applications.This assay gives ratiometric, inversely proportional values of viability and cytotoxicity (Figure 4.15) that are useful for normalizing data to cell number. Also, this reagent is compatible with additional fluorescent and luminescent chemistries. - [Read Determining Number of Live and Dead Cells in Cell Population: Cytotoxicity Assay Protocol]

Dual Luciferase Reporter System Protocol (PROMEGA) - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/II.%20Eukaryotes/F.%20Luciferase%20Assays/2b.Dual%20luciferase.pdf

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol for dual luciferase reporter system. Includes: Transfections; Cell Lysis; Reagent Preparation; Luminescence measurement; Wash injectors. - [Read Dual Luciferase Reporter System Protocol (PROMEGA)]

Dual Luciferase Assay System. Promega. PDF - http://www.promega.com/tbs/tm040/tm040.pdf

Category: Luciferase and Dual Luciferase Assays

Dual Luciferase Assay System. Promega. PDF - [Read Dual Luciferase Assay System. Promega. PDF]

Dye Terminator Cleanup for Automated DNA Sequencing Reactions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4101

Category: DNA Protocols: DNA Sequencing Protocols

Protocol describes a simple procedure for purifying sequencing reactions using the MagneSil particles from Promega. - [Read Dye Terminator Cleanup for Automated DNA Sequencing Reactions Protocol]

Eukaryotic In Vitro Translation Systems - http://www.promega.com/paguide/chap5.htm#title2

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Provides information on specific parameters you need to be aware of when using eukaryotic in vitro translation systems. Includes: DNA Template Considerations; Protein Labeling; Non-Radioactive Protein Labeling. - [Read Eukaryotic In Vitro Translation Systems]

Fan Lab Bisulfite Treatment of Genomic DNA - http://www.genetics.ucla.edu/labs/fan/Protocols_Bisulfite.htm

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

A detailed protocol for bisulfite treatment of DNA. DNA is first digested with a restriction enzyme. DNA is denauted at 97C for 5min. Bisulfite protocol is included. Also DNA purification using the Promega Wizard DNA Cleanup kit in indicated. Fan Lab. UCL - [Read Fan Lab Bisulfite Treatment of Genomic DNA]

Homogeneous Caspase 3-7 Assay Protocol - http://www.promega.com/tbs/tb295/tb295.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Homogeneous caspase-3/7 assay. Includes: Detection of Caspase-3/7 Activity in Cell Culture; Detection of Caspase-3 or -7 Activity in Purified Caspase Preparations; Positive and Negative Cell Culture Controls. - [Read Homogeneous Caspase 3-7 Assay Protocol]

Ligation with GUS vector and Transformation - http://www.owlnet.rice.edu/~bios311/bios311/bios312/312day3.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

Ligation with GUS vector and Transformation. Chemical Transformation of E. coli. Ligation Procedure for LigaFastâ„¢ Rapid DNA Ligation System (Promega). B. Beason, Rice University. - [Read Ligation with GUS vector and Transformation]