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Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol - http://www.genome.wisc.edu/functional/tnmutagenesis.htm

Category: E Coli Protocols: E coli Genetics Protocols

Protocol for systematic mutagenesis of E.coli K-12 MG1655 ORFs. Includes: Mutagenesis Project Progress; Construction of Sce-poson mutants; Mutagenesis of Essential Genes; Gene Gorging. - [Read Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]

Whole-Mount DAPI Staining and Measurement of DNA Content in Plant Cells - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4684

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

During development many plant cells undergo endoreduplication, whereby ploidy increases to a multiple of the normal 2C content. For eg., trichome development is accompanied by an increase in ploidy to 32C, indicating that trichome cells undergo four rounds of endoreduplication. Protocol describes DNA levels, and hence developmental progress in the corresponding cells, are measured by staining the DNA with a fluorescent marker and then quantifying the fluorescence of individual nuclei. - [Read Whole-Mount DAPI Staining and Measurement of DNA Content in Plant Cells]