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CALCIUM PHOSPATE TRANSFECTION OF HUMAN CELLS - http://omrf.ouhsc.edu/~frank/CAPO4TRANSFECT.html

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Simple Transfection using Calcium chloride and phosphate.Dr. Frank. Arthritis and Immunology Program, Oklahoma City. Medical Research Foundation. - [Read CALCIUM PHOSPATE TRANSFECTION OF HUMAN CELLS]

Electroporation of Eukaryotic Cells - http://omrf.ouhsc.edu/~frank/EPOREUK.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

The protocol uses a Bio-Rad Gene Pulser II apparatus to electroporate DNA into human cell lines. Dr. Frank, Arthritis and Immunology Program, Oklahoma City Medical Research Foundation - [Read Electroporation of Eukaryotic Cells]

ImageJ - Image Processing and Analysis in Java - http://rsb.info.nih.gov/ij/

Category: Molecular Imaging

ImageJ - Image Processing and Analysis in Java. Useful program for MacOs MacosX and PC. Analysis includes: Measure area, mean, standard deviation, min and max of selection or entire image. Measure lengths and angles. Use real world measurement units s - [Read ImageJ - Image Processing and Analysis in Java]

Laboratory Safety Program - http://www.uwm.edu/Dept/EHSRM/LAB/index.html

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Program - Emergency Preparedness, Autoclave Safety, Biosafety, Carcinogens, Chemical Hygiene Plan, Glass Disposal, Fire Safety, Eyewash and Safety Shower Information, Fume Hood Procedures and Practices. University of Wisconsin - Milwaukee. - [Read Laboratory Safety Program]

Maintenance of the AutoMACS Cell Sorter Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000017.pdf?pid=PP00000017

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

This protocol has been established to maintain the efficient use of the AutoMACS cell sorter during periods of heavy use (e.g., three preparations of splenocytes from 16 spleens per week). Includes: Running the SAFE Clean Program; Clearing Debris. - [Read Maintenance of the AutoMACS Cell Sorter Protocol]

PCR Program Design - http://info.med.yale.edu/genetics/ward/tavi/p08.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

The requirement of an optimal PCR reaction is to amplify a specific locus without any unspecific by-products. Therefore, annealing needs to take place at a sufficiently high temperature to allow only the perfect DNA-DNA matches to occur in the reaction. P - [Read PCR Program Design]

PCR Program Design - http://info.med.yale.edu/genetics/ward/tavi/p08.html

Category: PCR Protocols: PCR Optimization

Preparation of primary cultures of rat hepatocytes (or freshly isolated hepatocytes) - http://www.surgery.usc.edu/divisions/hep/research-cellculture.html

Category: Cell Biology and Cell Culture: Hepatocyte Cell Culture

USC Liver Transplant Surgery Program and Center for Liver Disease. Preparation of primary cultures of rat hepatocytes (or freshly isolated hepatocytes). - [Read Preparation of primary cultures of rat hepatocytes (or freshly isolated hepatocytes)]

Radiation Safety Manual - http://www.cdc.gov/OD/ohs/manual/radman.htm

Category: Radioactivity: Radioaction Safety

Administration of the Radiation Safety Program, , Physical Security of Radioactive Materials, "As Low as Reasonably Achievable" (ALARA) Philosophy, Authorized Users, Radiation Workers , Permitted Workers, Training, Procurement, Inventory and Records, Personnel Monitoring, Radiation Exposure During Pregnancy, Laboratory Supplies and Equipment, Bioassay, Labeling, Surveys, Radioactive Waste Disposal. U.S. Department of Health and Human Services, CDC, Atlanta, GA. - [Read Radiation Safety Manual]

SNP Restriction Digestion Comparison - http://insilico.ehu.es/restriction/two_seq/

Category: DNA Protocols: SNP Protocols

Compare restriction patterns of two sequences for SNP/Mutation detection. Online program that compares restriction enzyme digest of DNA and finds the differences between two sequences. - [Read SNP Restriction Digestion Comparison]

Spectrophotometric Measurement of Nucleic Acids' Concentration Tool - http://molbiol.ru/eng/scripts/01_03.html

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Spectrophotometric Measurement of Nucleic Acids' Concentration Tool. This bioinformatic program help calculate the concentration of nucleic acids according to optical density (including DNA, RNA, oligonucleotides). Zbio - [Read Spectrophotometric Measurement of Nucleic Acids' Concentration Tool]

Transformation of Bacteria - http://education.llnl.gov/bep/science/10/tTrans.html

Category: Microbiology: Bacterial Methods E.Coli Protocols

Transformation of Bacteria. Biotechnology Education Program - [Read Transformation of Bacteria]

Articles (1-2 of 2)

Microinjection Pipette Pulling Protocol

A simple protocol for microinjection pipette pulling.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

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