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Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9061

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol described here produces chromatin with regularly spaced nucleosomes having physiological nucleosome repeat lengths; something that can be difficult to achieve with purified components. In addition, chromatin assembled with the S-190 Chromatin Assembly Extract contains the ATP-dependent chromatin remodeling factors necessary for efficient transcription. - [Read Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl]

Hanahan Competent Cell Protocol. - http://www.rockefeller.edu/labheads/vosshall/protocols/Hanahan.pdf

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Hanahan Competent Cell Protocol. PROTOCOL PRODUCES ABOUT 160 X 250ul ALIQUOTS OF COMPETENT CELLS. Vosshall. Rockefeller. - [Read Hanahan Competent Cell Protocol.]

Hydroxylamine Mutagenesis of Plasmid DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4156

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol produces mutagenized DNA that can be used for transformation of either Escherichia coli or yeast. - [Read Hydroxylamine Mutagenesis of Plasmid DNA Protocol]

Phage Plate Stock Lysates Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda5.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml. - [Read Phage Plate Stock Lysates Protocol]

Polycrystalline thin films Sample Preparation Protocol MALDI - http://prowl.rockefeller.edu/methods/thinfilm.htm

Category: Mass Spectrometry Protocols

This method of sample preparation produces a uniform layer of very small crystals on the mass spectrometer's sample stage that are mechanically well adhered to the substrate. It is a variant of the dried droplet method. PROWL - [Read Polycrystalline thin films Sample Preparation Protocol MALDI]

Protocol for In Vitro Transcription of RNA - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3DSB4P24S4HQQQ3R3FQLMCFEWHUWBNSIV0?id=p340

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol produces RNA transcripts from a cloned gene that can then be used in an in vitro translation assay. - [Read Protocol for In Vitro Transcription of RNA]

Seeded films (Vorm-Roepstorff) MALDI Protocol - http://prowl.rockefeller.edu/methods/seeded.htm

Category: Mass Spectrometry Protocols

This sample preparation method is a direct replacement for the dried droplet technique. It produces more uniform samples than the dried-droplet technique and the crystals are better adhered to the sample stageâ€™s surface. MALDI Sample preparation. PROWL - [Read Seeded films (Vorm-Roepstorff) MALDI Protocol]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. The calcium phosphate method can be used to transfect neurons that have already been growing on coverslips in vitro. Transfected cells suitable for imaging can be obtained in cultures up to 15 days in vitro. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.