User Panel

My Panel

Bookmark Science Articles

procedure Protocols

Search results for: procedure

Protocols » Search Results

Featured Search Results Links

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Search Results Protocol Links Sort by: Hits | Alphabetical

Competitive ELISA Procedure - http://www.nottingham.ac.uk/~mbzspd/methods/Competitive_ELISA.html

Category: ELISA Protocols

Competitive ELISA Protocol. Jill Fergusson, University of Nottingham. - [Read Competitive ELISA Procedure]

Procedure for Transfection of Mammalian Cells Using Lipofectamine - http://www.research.umbc.edu/~jwolf/m10.htm

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Lipofectamine transient tranfection of mammalian cells in IMDM cell media. Julie B. Wolf, UMBC. - [Read Procedure for Transfection of Mammalian Cells Using Lipofectamine]

DNA Extraction From Blood Leukocytes - http://geneticslab.topcities.com/DNAextraction.htm

Category: DNA Protocols: DNA Extraction Protocols

DNA extracted from peripheral blood leucocytes using 3mls of whole blood. Modification of a salting out procedure as described by Miller et al., (1988). The DNA Laboratory, Medical School, Malta. - [Read DNA Extraction From Blood Leukocytes]

DNA digestion protocol & hints PDF - http://www.crypto.wustl.edu/Protocols%20PDFs/RE%20digests%20(HL).pdf

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

DNA digestion protocol & hints. Procedure and tips for restriction digestion. Doering laboratory, Department Molecular Microbiology, Washington University Medical School. - [Read DNA digestion protocol & hints PDF]

Sandwich ELISA Procedure - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Biochemistry/sELISA.html

Category: ELISA Protocols: Sandwich ELISA Assay Protocols

Sandwich ELISA Procedure. Kitto Lab, Univ. Texas at Austin - [Read Sandwich ELISA Procedure]

Protocol for Real-Time RT-PCR Using SYBR Green - http://pga.mgh.harvard.edu/primerbank/PCR_protocol.html

Category: Real Time PCR

A detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Wang et al 2003. - [Read Protocol for Real-Time RT-PCR Using SYBR Green]

HPLC Protocol - http://tesla.ccrc.uga.edu/group_pages/protocols/downloads/HPLCProtocol.doc

Category: HPLC Protocols

HPLC Protocol. A general HPLC procedure to get you started on High Performance Liquid Chromatography. - [Read HPLC Protocol]

AFLP protocol - http://www.msu.edu/user/hazensam/aflp/AFLPprotocolMSU.html

Category: PCR Protocols: AFLP PCR

Restriction Digestion of Genomic DNA , Adapter preparation, Ligation of Adapters. Pre amplification Reactions. Gel electrophoresis, Silver Staining. AFLP Protocol and procedure. - [Read AFLP protocol]

Cell Lysate Extracts - http://www.upstate.com/misc/protocol_detail.q.prot.e.CellLysate_Prep.a.name.e.Cell_Lysate_Extracts-General_Protocols

Category: Protein Protocols: Protein Extraction From Cells

Cell Lysate Extracts. Great protocols for cell lysis preparation from a variety of cell types. There are numerous methods of cell stimulation and lysis. For a given protein, Upstateâ€™s Laboratories determine the specific treatment upon initial testing of its products. It is important to select the correct cell line, stimulation procedure (if any), and lysis protocol. Upstate. - [Read Cell Lysate Extracts]

ELISA Procedure for Measuring Serum Antibody Titer - http://www.komabiotech.com/technical/protocol/ELISA.htm

Category: ELISA Protocols

Method and ELISA Procedure for Measuring Serum Antibody Titer. Komabiotech - [Read ELISA Procedure for Measuring Serum Antibody Titer]

DNA Extraction from Cheek Cells - http://geneticslab.topcities.com/cheekcells.htm

Category: DNA Protocols: DNA Extraction Protocols

Cheek cells obtained by rinsing the mouth commercial mouth wash solution. Mouth wash is then discarded into a sterile conical tube and sent to the lab. Based on Salting out procedure. DNA Laboratory, Medical School, Malta. - [Read DNA Extraction from Cheek Cells]

Preparing Chemically Competent Cells - http://openwetware.org/wiki/Preparing_chemically_competent_cells

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Detailed protocol Preparation and Steps for E.Coli Competent Cells procedure. Open Net Ware. - [Read Preparing Chemically Competent Cells]

AGAROSE GEL PROCEDURE - http://www.stolaf.edu/people/muth/agarosegels.html

Category: Molecular Biology Protocols: Agarose Gel Preparation

AGAROSE GEL PROCEDURE in pictures. Biochemistry at St. Olaf College Muth Lab - [Read AGAROSE GEL PROCEDURE]

TCA/Acetone Precipitation (Large Scale) PDF - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000148.pdf?pid=PP00000148

Category: Protein Protocols: Protein Precipitation Procols

A protein precipitation procedure used to precipitate protein from cell lysates. Allows for optimal protein recovery and accurate assays. The Signaling Gateway - [Read TCA/Acetone Precipitation (Large Scale) PDF]

Simple Western Blot Protocol - http://www.prosci-inc.com/Antibody/Western-Blot-Procedure.html

Category: Protein Protocols: Western Blot Protocol

Simple Western Blotting Protocol ProSci. - [Read Simple Western Blot Protocol]

ChIP ping for Dummies - http://mcardle.oncology.wisc.edu/sugden/Protocols/html%20files/ChIPping%20for%20Dummies.htm

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Very nice and detailed ChIP assay protocol procedure. Blocking Staph A cells, Crosslinking cells, Chromatin preparation from cell pellets, sonication, blocking. Sugden Laboratory - [Read ChIP ping for Dummies]

Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html

Category: DNA Protocols: TOPO Cloning

Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]

MEF Mouse Embryonic Fibroblast Protocols - http://ink.primate.wisc.edu/~thomson/protocol.html#harvest

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

MEFs. Thompson Lab Stem Cell Methods and Procedure. Irradiation and plating MEFs, Splitting MEF cells, Thawing MEF, Freezing and Harvestion MEF cells. - [Read MEF Mouse Embryonic Fibroblast Protocols]

Yeast Genomic DNA Isolation Procedure - http://www.upstate.edu/biochem/amberg/protocols/yeast_genomic_DNA.html

Category: DNA Protocols: DNA Extraction Protocols

Yeast Genomic DNA Isolation David Amberg, Upstate Medical University. - [Read Yeast Genomic DNA Isolation Procedure]

Western Blot - http://www.biochem.emory.edu/labs/acorbe2/protocols9.html

Category: Protein Protocols: Western Blot Protocol

Simple Procedure for Western Blotting. Corbett Lab. - [Read Western Blot]

Troubleshooting the immunoprecipitation procedure IP and IB - Roche - http://www.roche-applied-science.com/PROD_INF/MANUALS/epitope/p66.pdf

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Troubleshooting the immunoprecipitation procedure Roche. Tagged proteins not visible or only weakly visible on Western blot. High background or many nonspecific bands on gel or blot membrane. - [Read Troubleshooting the immunoprecipitation procedure IP and IB - Roche]

Lowry Protein Assay - http://www.animal.ufl.edu/hansen/protocols/LOWRY.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay. The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-275 (1951)]. Under alkaline conditions, copper complexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent binds to the protein. Bound reagent is slowly reduced and changes color from yellow to blue. P.J. Hansen, Dept. of Animal Sciences, University of Florida. - [Read Lowry Protein Assay]

ChIP TIPS PDF - http://www.upstate.com/img/pdf/ChIP_Protocol_thin_v2.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Prepare Chromatin, Immunoprecipitate, Wash, Elute, IP Procedure. UpState. - [Read ChIP TIPS PDF]

The Procedure for Optimized ELISA Protocol - http://www.pall.com/34445_4769.asp

Category: ELISA Protocols

The Procedure for Optimized ELISA Protocol. PALL Corp. - [Read The Procedure for Optimized ELISA Protocol]

Methanol-Chloroform Precipitation of Proteins - http://www.healthsystem.virginia.edu/internet/biomolec/methanolchloroform.cfm

Category: Mass Spectrometry Protocols

Methanol-Chloroform Precipitation of Proteins. A procedure for precipitating proteins from solution, including detergent solutions, is from Wessel and FlÃ¼gge (1984). Univ. Virginia. - [Read Methanol-Chloroform Precipitation of Proteins]

Articles (1-10 of 12)

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.