principles Protocols

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol describes typical methods that are used to propagate and purify AAV vectors for experiments both in vitro and in vivo. Includes: Principles of the Triple Plasmid Transfection System; Plasmids; Transfection and Extraction of Virus; Purification of the AAV vector. - [Read A Protocol for AAV Vector Production and Purification]

Category: Cloning Protocols: Vector Protocols

Protocol is used to propagate and purify AAV vectors for experiments both in vitro and in vivo. Includes: Principles of the Triple Plasmid Transfection System; Plasmids; Transfection and Extraction of Virus; Purification of the AAV vector. - [Read AAV Vector Production and Purification Protocol]

Category: Immunology: Antigen Retrieval

Antigen Retrieval. Principles and Technique. A handbook of Immunochemical Staining Methods and techniques. Marc Key - [Read Antigen Retrieval PDF]

Category: Neuroscience Protocols: Patch Clamping Protocols

Principles behind the techniques: channel specific techniques. - [Read Channel Specific Techniques]

Category: Protein Protocols: Immunoprecipitation Protocols

Great Tips on IP - Bart's Cookbook - [Read General Principles of Immunoprecipitation - Great Tips]

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Describes the basic principles of in situ hybridization and advantages and disadvantages of different methodologies that can be used. Includes: Probe Selection; Probe Generation; Probe Labels; Fixation of Tissue; Hybridization and Washing; Control Procedures. - [Read In situ Hybridization]

Category: Radioactivity: Liquid Scintillation Counting

Excellent guide for Liquid Scintillation Counting. Includes protocols and methods for counting gel slices, SPECIAL SAMPLE PREPARATION PROTOCOLS: TLC Plates, protocol for Counting Samples on Cellulose-ester, Filters (MilliporeTM filters), Counting Tissue radioacitivity, Counting 14CO2, Samples in Polyacrylamide Gels. National Diagnostics. National Diagnostics Laboratory Staff. - [Read Principles and Applications of Liquid Scintillation Counting PDF]

Category: Animal Research Techniques

Legal responsibilities, Recognition and treatment of pain, Anesthetic monitoring, stages and planes of general anesthesia, injecting, inhalation, species-specific, anesthetic emergencies, controlled substances. Marilyn J. Brown UCSB - [Read Principles of Anesthesia and Analgesia: ESSENTIALS FOR ANIMAL RESEARCH]

Category: Animal Research Techniques

Facilities, equipment, sterilization, steam, gas, liquid, animal research, preparation of the animal, surgical pack, surgeon preparation. - [Read Principles of Aseptic Technique: ESSENTIALS FOR ANIMAL RESEARCH]

Category: RNA Protocols: cDNA Libraries Library

General principles and procedures. Good tips. Atle M. Bones Lab. - [Read Screening of cDNA and genomic libraries.]

Category: Real Time PCR: Real Time PCR Information

The Usefulness of Real-time PCR in Immunogenetics. Principles, technique, applications of Real time pcr. Provenzano et al., Scientific 2001 Communications. - [Read The Usefulness of Real-time PCR in Immunogenetics]

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

A. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial DNA (the transfer, or "T," DNA) into the plant cell genome. The Ti plasmid also carries many of the transfer functions for mobilizing the T-DNA. This article provides a brief discussion of the principles of T-DNA transformation, including consideration of T-DNA vectors and their hosts. - [Read Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol]