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How to Name and Symbolize Genes at Previously Unnamed Loci - http://www.fgsc.net/neurosporaprotocols/How%20to%20name%20and%20symbolize%20genes%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to name and symbolize genes at previously unnamed loci. - [Read How to Name and Symbolize Genes at Previously Unnamed Loci]

Purification of Protein Using Dye-Ligand Affinity Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4213

Category: Chromatography Protocols: Dye Ligand Affinity Chromatography Protocols

Protocol describes a general method for the purification of protein using dye-ligand affinity chromatography. The effectiveness of this protocol is dependent upon the reader having previously determined the optimal dye and buffer conditions to use for binding and eluting the protein(s) of interest. - [Read Purification of Protein Using Dye-Ligand Affinity Chromatography Protocol]

Simultaneous Analysis of DNA Content and Surface Immunophenotype Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/ethanol.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

In an attempt to accurately measure DNA content with simultaneous preservation of cell surface markers, we have utilized gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association. For some cell types, the presence of apoptotic cells based on reduced DNA content can also be detected. One such technique employs the addition of ethanol to cells previously resuspended in high concentrations of fetal bovine serum... - [Read Simultaneous Analysis of DNA Content and Surface Immunophenotype Protocol]

SKY/FISH of Previously G-Banded Slides Protocol - http://www.riedlab.nci.nih.gov/publications/SKY-FISH%20G_Banded_slides.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for SKY/FISH of previously G-banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization. - [Read SKY/FISH of Previously G-Banded Slides Protocol]

SKY/FISH of Previously G-Banded Slides Protocol - http://www.riedlab.nci.nih.gov/publications/SKY-FISH%20G_Banded_slides.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for SKY/FISH of previously G-Banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization. - [Read SKY/FISH of Previously G-Banded Slides Protocol]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.