preuss Protocols

Category: Microbiology: Mini-Prep Plasmid Purification

Harvesting and Lysis of Bacteria. Mini-prep plasmid purification using Solution I, Solution II and Solution III. The Preuss Lab. Univ. Chicago. - [Read Alkaline Lysis Mini-Prep Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Basic PCR Protocol. (per Adam 08/12/04). - [Read Basic PCR Protocol]

Category: PCR Protocols

Basic PCR Protocol. (per Adam 08/12/04). - [Read Basic PCR Protocol]

Category: RNA Protocols: RT-PCR and cDNA synthesis

Simple RT-PCR Protocol. University of Chicago The Preuss Lab - [Read Simple RT-PCR Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

TAIL PCR Protocol. TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 reactions are the AD (Arbitrary Degenerate) primers, border primers, and DNA from the T-DNA - [Read TAIL PCR Protocol]

Category: PCR Protocols

TAIL PCR Protocol. TAIL is a series of reactions that are intended to map where a T-DNA (transfer DNA) has inserted within the genome. The main components of the 3 reactions are the AD (Arbitrary Degenerate) primers, border primers, and DNA from the T-DNA - [Read TAIL PCR Protocol]

Category: DNA Protocols: TOPO Cloning

Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]