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COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL - http://users.ugent.be/~fspelema/neubla/protocol/cgh.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

SAVING OF DAPI-IMAGES, SLIDE PRETREATMENT, PROBE PREPARATION, PROBE DETECTION, washing blocking detection, Counterstaining with DAPI. Institute of Pathology,Humboldt-University of Berlin - [Read COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL]

Detection of HPV 11 DNA in Paraffin-Embedded Laryngeal Tissue with a DIG-Labeled DNA Probe Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_143-148.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for detection of HPV 11 DNA in paraffin-embedded laryngeal tissue with a DIG-labeled DNA probe. Includes: Probe preparation; Pretreatment of slides; Preparation of tissue sections; Hybridization; Washes; Detection of hybrids. - [Read Detection of HPV 11 DNA in Paraffin-Embedded Laryngeal Tissue with a DIG-Labeled DNA Probe Protocol]

In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_78-94.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for In situ hybridization to human metaphase chromosomes using DIG-, biotin-, or fluorochrome-labeled DNA probes and detection with fluorochrome conjugates. Includes: Pretreatment of metaphase spreads on slides; Denaturation and hybridization; Single color fluorescent detection with immunological amplification; Multicolor fluorescence in situ hybridization (Multicolor FISH); Results obtained with human metaphase chromosome spreads. - [Read In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols

Protocol for pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

SKY/FISH of Previously G-Banded Slides Protocol - http://www.riedlab.nci.nih.gov/publications/SKY-FISH%20G_Banded_slides.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for SKY/FISH of previously G-banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization. - [Read SKY/FISH of Previously G-Banded Slides Protocol]

SKY/FISH of Previously G-Banded Slides Protocol - http://www.riedlab.nci.nih.gov/publications/SKY-FISH%20G_Banded_slides.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for SKY/FISH of previously G-Banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization. - [Read SKY/FISH of Previously G-Banded Slides Protocol]

Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_220-228.pdf

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for whole mount fluorescence in situ hybridization (FISH) of repetitive DNA sequences on interphase nuclei of the small cruciferous plant Arabidopsis thaliana. Includes: Seed sterilization and germination; Tissue fixation; Labeling of the probe DNA; Pretreatment; In situ hybridization; Pre-absorption of antibodies; Posthybridization washes; Immunocytochemical detection; Direct detection; Indirect detection; Staining and mounting; Fluorescence microscopy. - [Read Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase]