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Cytogenetic Protocol Blood Preps - http://www.jax.org/cyto/blood_preps.html

Cytogenetic protocol for blood preps. Includes: Preparation of materials; Culture Procedure - Use aseptic technique throughout procedure until harvest; Harvest; Slide making; Collecting blood by tail vein method; PHA Dose Response. - [Read Cytogenetic Protocol Blood Preps]

Drosophila Single-Fly DNA Preps For PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafly.html

Category: Drosophila Protocols: Drosophila Nucleic Acids Protocols

Protocol for Drosophila single-fly DNA preps for PCR. Includes: DNA Preparation Protocol; DNA Preps from many individual flies; Inverse PCR. - [Read Drosophila Single-Fly DNA Preps For PCR Protocol]

Phage Plate Stock Lysates Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda5.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml. - [Read Phage Plate Stock Lysates Protocol]

Quick DNA Plasmid Prep Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D4.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for quick DNA plasmid prep. Protocol gives very clean plasmid preps for restriction digests and cloning. However, due to the alkaline lysis step, the DNA is often nicked and may not give exceptional sequence data. - [Read Quick DNA Plasmid Prep Protocol]

Small Scale DNA Preps for Neurospora crassa Protocol - http://www.fgsc.net/fgn/irelan.html

Category: Fungi Protocols: Neurospora Protocols

DNA isolation method yields an average of 0.6 micrograms of genomic DNA that is suitable for Southern analysis or PCR. Starting with fresh mycelium, 20 to 40 samples can be processed in approximately two hours. Better yields (about 5 micrograms) may be obtained by suspending approximately 100 microliters of ground lyophilized mycelium in 500 microliters of isolation buffer and following the protocol. - [Read Small Scale DNA Preps for Neurospora crassa Protocol]

Tail DNA Preps for Genomic Southerns Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp10.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for tail DNA preps for genomic southerns. - [Read Tail DNA Preps for Genomic Southerns Protocol]

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Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.