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Preparing Stocks of Bacteriophage {lambda} by Plate Lysis and Elution Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3963

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

A reliable method to prepare plate lysates and to recover infectious bacteriophages by elution from the top agar. - [Read Preparing Stocks of Bacteriophage {lambda} by Plate Lysis and Elution Protocol]

Preparing Stocks of Bacteriophage {lambda} by Small-scale Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3971

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

High-titer stocks of bacteriophage {lambda} are easily prepared by infecting small-scale bacterial cultures. - [Read Preparing Stocks of Bacteriophage {lambda} by Small-scale Liquid Culture Protocol]

Preparing the Aggregation Plate Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4420

Category: Mouse Protocols: Assembling Chimeras Mouse Protocols

Protocol describes the preparation of aggregation plates, which will then be used for the production of aggregation chimeras. - [Read Preparing the Aggregation Plate Protocol]

Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol - http://www.fgsc.net/neurosporaprotocols/VM%20vacuole%20procedure.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa (4 liter prep, easily scaled up to 10, 12, 24, or 30 l). - [Read Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol]

PROTOCOL FOR SDS-PAGE - http://www.fiu.edu/~animals/protocols/sds_page.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

PROTOCOL FOR SDS-PAGE. Immuno-Physiology Laboratory, Florida International University. Includes: Primary considerations, preparing samples, loading samples, running gels, and quantitative staining. - [Read PROTOCOL FOR SDS-PAGE]

Protocols for Mass Spectrometry Analysis Preparation - http://www.st-andrews.ac.uk/~bmsmspf/protocols.htm#manual

Category: Mass Spectrometry Protocols

Protocols for Mass Spectrometry Analysis Preparation. Preparing gel slices for PMF or MS/MS , mass spec data interpretation, Submitting proteins in solution, Interpretation of LC-MS/MS data. BMS MASS SPECTROMETRY AND PROTEOMICS FACILITY - [Read Protocols for Mass Spectrometry Analysis Preparation]

Streaking Lambda Phages Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda12.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Phage are streaked onto a medium to obtain an independent isolate prior to preparing a new lysate. This is done to reduce the likelihood of working with lysates which have become contaminated, and/or have accumulated mutations. - [Read Streaking Lambda Phages Protocol]

Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3869

Category: PCR Protocols: cDNA Synthesis Protocols

Protocol describes the preparation of subtracted cDNA probes by hybridization to an mRNA driver, followed by purification of the single-stranded radiolabeled cDNA by hydroxyapatite chromatography. Before preparing the probe, it is a good idea to have filters (which contain the cDNA library to be screened) ready to hybridize. - [Read Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer Protocol]

Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000182.pdf?pid=PP00000182

Category: Transfection Protocols

The following procedure is for simultaneous transfection and plating of RAW 264.7 cells. This protocol results in approximately 50% to 70% cell viability, and of those viable cells, 20% to 40% are transfected when using pEYFP-N1 from Clontech. Include: Procedure for Splitting Cells before Transfection; Procedure for Preparing Lipofectamine 2000 and DNA; Preparation of RAW 264.7 Cells for Transfection. - [Read Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol]

Transformation of E. coli by Electroporation Protocol - http://userpages.umbc.edu/~jwolf/m7.htm

Category: E Coli Protocols: E coli Transformation Protocols

Protocol for the transformation of E. coli by electroporation. Includes: Preparation of E. coli cells for electroporation; Preparing DNA for Electroporation. - [Read Transformation of E. coli by Electroporation Protocol]

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Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

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