For low-resolution work, cells to be used for staining can be grown directly on regular tissue-culture dishes. It is a convenient method that does not require much preparatory work. - [Read Growing Adherent Cells Directly on Tissue Dishes Protocol]
The key preparatory steps. In-situ RT-PCR. Fixative. Protease Digestion. DNase digestion. One-step in-situ RT PCR Protocol. Gerard J. Nuovo - [Read RT In Situ PCR - Foundation of Success]