preparative Protocols

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Fixation and Fixatives - A great guide - http://home.primus.com.au/royellis/fix.htm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation is to preserve cells and tissue constituents in as close a life-like state as possible and to allow them to undergo further preparative procedures without change. Fixation arrests autolysis and bacterial decomposition and stabilises the cellular and tissue constituents so that they withstand the subsequent stages of tissue processing. Great detailed guide with protocols. Anthony S-Y Leong. - [Read Fixation and Fixatives - A great guide]

Immunoblotting: Preparing Immunoprecipitated Proteins Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4300

Category: Western Blot Protocols

Protein immunoprecipitation can be a useful preparative step for immunoblotting. For very rare proteins, the protein of interest can be purified and concentrated by standard immunoprecipitation techniques before immunoblotting. In addition, protein-protein interactions can be tested with an immunoprecipitating antibody that is specific for one protein of a complex and an immunoblotting antibody that is specific for a second member of the complex. - [Read Immunoblotting: Preparing Immunoprecipitated Proteins Protocol]

Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Preparative Reactions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3979

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

The results of the pilot experiment (Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Pilot Reactions) are used to establish conditions for partial digestion of eukaryotic DNA in the large-scale reactions described here. - [Read Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Preparative Reactions Protocol]

Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infection - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3771

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

This rapid method is used to screen bacteriophage {lambda}gt11 clones for the production of immunodetectable fusion proteins. After optimizng the conditions of infection and induction, the method can be used to produce preparative amounts of a fusion protein. - [Read Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infection]

Restriction Enzyme Digestion of DNA - http://www.facstaff.bucknell.edu/pizzorno/327-lab4.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Digesting DNA with Restriction Endonucleases introduction. Analytical and Preparative reaction mixes. All about restriction enzymes. Pizzorno, BIOL 327. - [Read Restriction Enzyme Digestion of DNA]

Use of PCR to Prepare a Double-Stranded DNA Library Encoding Random Peptides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4135

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

PCR is used as a preparative tool for the synthesis of a high-complexity double-stranded DNA library. In the example presented here, a mixture of synthetic oligonucleotides is used to synthesize a random peptide NNK library, where K is either T or G. The exclusion of A and C nucleotides at the third position decreases the occurrence of stop codons but still allows codons for all 20 amino acids. - [Read Use of PCR to Prepare a Double-Stranded DNA Library Encoding Random Peptides Protocol]