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The siRNA user guide - http://www.rockefeller.edu/labheads/tuschl/sirna.html

Selection of siRNA duplexes from the target mRNA sequence, Preparation of siRNA duplexes, Useful siRNA reagent combinations, Transfection of siRNA duplexes, Sequences of siRNA duplexes used in our studies. Tuschl Lab. - [Read The siRNA user guide]

The siRNA User Guide - http://www.rockefeller.edu/labheads/tuschl/sirna.html

Category: RNAi Technology Protocols

The siRNA user guide. Includes: Selection of siRNA duplexes from the target mRNA sequence; Preparation of siRNA duplexes; Useful siRNA reagent combinations; Transfection of siRNA duplexes; Sequences of siRNA duplexes used in our studies. - [Read The siRNA User Guide]

Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells Protocol - http://www.natureprotocols.com/2006/11/17/thymosin_4_t4induced_outgrowth.php

Category: Mouse Protocols: Mouse Cell Culture Protocols

Protocol for thymosin β4 (Tβ4)-induced outgrowth and differentiation of vasculogenic precursor cells from adult epicardium. Includes: Preparation of EPDCs; Characterisation of EPDC Phenotypes by Immunofluorescence. - [Read Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells Protocol]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Category: Microscopy Protocols: In Vivo Imaging Protocols

Sophisticated fluorescence microscopy methods & equipment, now allow cellular events to be studied at high resolution in living material. The studying of living fly tissues presents unique difficulties in keeping the cells alive, introducing fluorescent probes, & imaging through thick hazy cytoplasm. This protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Category: Drosophila Protocols

Protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol]

Tips for Sample Preparation for MALDI mass spectrometry - http://www.cfgbiotech.com/proteomics/sample_prep.htm

Category: Mass Spectrometry Protocols

Tips for Sample Preparation for MALDI mass spectrometry. Sample quantity, Solvent, Protein/Peptide sample handling. U. Albany. CFG - [Read Tips for Sample Preparation for MALDI mass spectrometry]

Top Agar Preparation - http://rothlab.ucdavis.edu/protocols/M11.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Prepare Top Agar and pouring plates for top agar. John Roth, U.C Davis. - [Read Top Agar Preparation]

Total RNA isolation (Guanidinium Thiocyanate-Phenol-Chloroform Extraction) Protocol - http://www.flemingtonlab.com/Protocols/TotalRNAprep.pdf

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols

Protocol for total RNA isolation. This method is fast and works well for preparation of total RNA. We typically use total RNA for quantitative S1 nuclease analysis of endogenous (or in vitro) gene expression, and this method works well for this purpose. - [Read Total RNA isolation (Guanidinium Thiocyanate-Phenol-Chloroform Extraction) Protocol]

Transcript Profiling by Microarray Analysis Protocol. - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000019.pdf?pid=PP00000019

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited cDNAs. The procedure requires a minimum of 5 mg of purified total RNA as starting material. Includes: First Strand cDNA Synthesis; Second Strand cDNA Synthesis; cDNA Cleanup and Precipitation; In vitro Transcription; Cleanup and Quantification of in vitro Transcribed RNA; Fluorescent Labeling of the Target Samples. - [Read Transcript Profiling by Microarray Analysis Protocol.]

Transcript Profiling by Microarray Analysis—Agilent Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000019.pdf?pid=PP00000019

Category: Microarray Protocols: Microarray DNA Labeling Protocols

Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000182.pdf?pid=PP00000182

Category: Transfection Protocols

The following procedure is for simultaneous transfection and plating of RAW 264.7 cells. This protocol results in approximately 50% to 70% cell viability, and of those viable cells, 20% to 40% are transfected when using pEYFP-N1 from Clontech. Include: Procedure for Splitting Cells before Transfection; Procedure for Preparing Lipofectamine 2000 and DNA; Preparation of RAW 264.7 Cells for Transfection. - [Read Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol]

Transformation of E. coli by Electroporation Protocol - http://userpages.umbc.edu/~jwolf/m7.htm

Category: E Coli Protocols: E coli Transformation Protocols

Protocol for the transformation of E. coli by electroporation. Includes: Preparation of E. coli cells for electroporation; Preparing DNA for Electroporation. - [Read Transformation of E. coli by Electroporation Protocol]

Transgenic - Universal Mouse Genotyping Protocol Using PCR - http://www.knockoutmousestation.com/mouse/detail/link-28.html

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Description:, Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. ... - [Read Transgenic - Universal Mouse Genotyping Protocol Using PCR]

Transient Transfection and Luciferase assay - http://www.natureprotocols.com/2006/10/27/transient_transfection_and_luc.php

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection into mammalian cells is a convenient way to over express and obtain protein expression. Protocol includes: Culture conditions; Transfection of experimental cells; Preparation of Mammalian Cell Lysate for Luciferase assay. - [Read Transient Transfection and Luciferase assay]

Transient Transfection and Luciferase Assay Protocol - http://www.natureprotocols.com/2006/10/27/transient_transfection_and_luc.php

Category: Reporter Gene Assays: Luciferase Assay Protocols

Ultimate His-UB Assay for Mammalian Cells - http://tanseylab.cshl.edu/pdf/His_Ub_mammals.pdf

Category: Protein Protocols: Protein Ubiquitination Protocols

Ultimate His-UB Assay for Mammalian Cells. Tansey Lab Protocols. William P. Tansey. PREPARATION OF NI-NTA-AGAROSE, HARVESTING THE TRANSFECTION., ELUTION OF HIS-TAGGED PROTEINS. - [Read Ultimate His-UB Assay for Mammalian Cells]

Universal Mouse Genotyping Protocol Using PCR - http://mgc.wustl.edu/protocols/pcr_genotyping.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. - [Read Universal Mouse Genotyping Protocol Using PCR]

Visualizing miRNAs in Adult Skeletal Muscle by Hybridization In Situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for visualizing miRNAs in adult skeletal muscle by hybridization in situ. Includes: Visualizing miRNAs in adult skeletal muscle by hybridization in situ; Probe preparation; Tissue preparation; Fixation; Deproteinization with Proteinase K; Acetylation of the tissue; Dehydration; Hybridization step; Post-Hybridization Washes (Step 1-6 should be performed on shaker); Signal Detection (Steps should be performed on a shaker); Post-Detection Washes. - [Read Visualizing miRNAs in Adult Skeletal Muscle by Hybridization In Situ Protocol]

Visualizing miRNAs in Adult Skeletal Muscle by Hybridization in situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php

Category: Cytogenetics Protocols: RNA In Situ Hybridization Protocols

Vitamin Mixtures for Plant Growth Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Vitamin_Mixtures.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for vitamin mixtures for plant growth media. Includes: Preparation of Solutions from Packaged Powder; Media Preparation Using Vitamin Solutions. - [Read Vitamin Mixtures for Plant Growth Media Protocol]

Western Blot Detailed (PDF) - http://www.its.caltech.edu/%7Ebjorker/Western_blot.pdf

Category: Protein Protocols: Western Blot Protocol

Protocol includes cell lysate preparation, SDS-PAGE gels, transfer, and blotting. (Bjorkman Group). - [Read Western Blot Detailed (PDF)]

Western Blot Protocol - http://www.sgul.ac.uk/depts/immunology/~dash/group/western.html

Category: Protein Protocols: Western Blot Protocol

Nice Tips on reducing sample viscosity, buffer preparation, and western blot protocol. Phil Dash. - [Read Western Blot Protocol]

Whole Mount In Situ Hybridization of Mouse Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/in_situ.html

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol for whole mount in situ hybridization of mouse. Incluses: Preparation of the probe; General Comments; Preparation of embryos; In Situ hybridization. - [Read Whole Mount In Situ Hybridization of Mouse Protocol]

Xenopus Egg CSF Extract Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1049

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol describes the preparation of Cytostatic Factor (CSF) from frog eggs. CSF can be used in in vitro spindle assembly assays. Includes: Frog injections; Preparation Before Starting the Extract Preparation; Extract Preparation. - [Read Xenopus Egg CSF Extract Preparation Protocol]

Xenopus Sperm Nuclei Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1050

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for Xenopus sperm nuclei preparation. Sperm Nuclei can be made and stored as a stock for later use with Cytostatic Factor Extracts. - [Read Xenopus Sperm Nuclei Preparation Protocol]

Articles (1-10 of 13)

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.