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Run- On Transcription Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p701

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Run-on transcription monitors the regulation of transcription in isolated nuclei. Protocol includes: Preparation of Nuclei, Run-On Transcription Reaction, Alkaline Hydrolysis of Run-Off Transcripts, Preparation of Filters and Hybridization. Solutions included: DNase Solution, Hybridization Mix, Denhardt Solution (100X) etc. - [Read Run- On Transcription Protocol]

SAPK / Jun kinase assays - http://kinase.uhnres.utoronto.ca/methods/SAPKassays.html

Category: Signalling Signal Transduction Protocols

SAPK/Jun kinase assays. Preparation of cell lysate, Substrate/Antibody, Lysate Interaction and buffer recipes. Woodgett Lab PMH. - [Read SAPK / Jun kinase assays]

SAPK/Jun Kinase Assays - http://kinase.uhnres.utoronto.ca/methods/SAPKassays.html

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol for SAPK/Jun kinase assays. Protocol includes information on: preparation of cell lysate, substrate/antibody, lysate interaction. - [Read SAPK/Jun Kinase Assays]

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) - http://www.mcb.uct.ac.za//sdspage.htm

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE). Ed Rybicki and Maud Purves. Dept Microbiology, University of Cape Town. PROTOCOL includes: Assembling Gel Apparatus, Resolving Gels, Stacking Gels, Electrophoresis buffer, Sample Preparation for Sds-page and Staining of Gels. - [Read SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)]

SDS-PAGE Sample Preparation of Adenovirus Infected Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1752

Category: Viral Protocols

Protocol for SDS-PAGE sample preparation of adenovirus infected cells. - [Read SDS-PAGE Sample Preparation of Adenovirus Infected Cells Protocol]

Seeded films (Vorm-Roepstorff) MALDI Protocol - http://prowl.rockefeller.edu/methods/seeded.htm

Category: Mass Spectrometry Protocols

This sample preparation method is a direct replacement for the dried droplet technique. It produces more uniform samples than the dried-droplet technique and the crystals are better adhered to the sample stageâ€™s surface. MALDI Sample preparation. PROWL - [Read Seeded films (Vorm-Roepstorff) MALDI Protocol]

Simultaneous Measurement of Intracellular CA2+ and Cell Surface Antigen Expression Protocol - http://cyto.mednet.ucla.edu/Protocols/Calcium%20Staining.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

INDO-1 loading and sample staining protocol for simultaneous measurement of intracellular CA2+ and cell surface antigen expression. Includes: Preparation of INDO-1; Preparation of Ionomycin. - [Read Simultaneous Measurement of Intracellular CA2+ and Cell Surface Antigen Expression Protocol]

Slide Preparation for Cytogenetics Protocol - http://info.med.yale.edu/genetics/ward/tavi/Cytom-slideprep.html

Category: Cytogenetics Protocols

Protocol for slide preparation for cytogenetics. Includes: Improved cell recovery by microcentrifugation; Slide storage; "Controlled" chromosome spreading; Dropping cells from a height does not improve spreading; GTG-banding. - [Read Slide Preparation for Cytogenetics Protocol]

Slide preparation for FISH and Cytogenetics - http://www.sanger.ac.uk/HGP/methods/cytogenetics/slide_preparation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Slide preparation for FISH and Cytogenetics. - [Read Slide preparation for FISH and Cytogenetics]

Slide Preparation for Laser Capture Microdissection (LCM) Protocol - http://cgap-mf.nih.gov/Protocols/Slides/SlideProtocols/SlidePrepforLCM.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols: Tissue Preparation for LCM Protocols

Protocol for slide preparation for laser capture microdissection (LCM) for subsequent DNA, RNA, and protein analysis. - [Read Slide Preparation for Laser Capture Microdissection (LCM) Protocol]

Slide Preparation for Manual Microdissection Protocol - http://cgap-mf.nih.gov/Protocols/Slides/SlideProtocols/SlidePrepManualMicrodissection.html

Category: Primary Cell Isolation and Culture Protocols

Slide Preparation for Manual Microdissection for Subsequent DNA, RNA, and Protein Analysis. Manual microdissection and subsequent molecular analysis can be carried out on slides stained using standard hematoxylin and eosin methods. However, if cell types that are (or are not) expressing a specific protein are required for a study, then more advanced slide preparation methods such as Immuno-LCM may be utilized. - [Read Slide Preparation for Manual Microdissection Protocol]

SNP Genotyping System - http://www.promega.com/tbs/tm053/tm053.pdf

Category: Genetics and Genomics: Genotyping Protocols

SNP Genotyping System. Includes: Protocol for Manual READIT® Assay in Multiwell Plates; Preparation of L/L Reagent; Preparation of Heating Block; CIAP/Exo Treatment; Preparation of Interrogation Probe Solutions; Template Denaturation; Sample Interrogation; Protocol for Manual Plate Reading Luminometers; Protocol for Injecting Plate Reading Luminometers; Protocol for Automated READIT® Assay with the Biomek® 2000 Instrument. - [Read SNP Genotyping System]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for sodium and potassium analysis by flow cytometry. Includes: Preparation of the dyes; Loading of the dyes; Flow Cytometric Analysis. - [Read Sodium and Potassium Analysis by Flow Cytometry Protocol]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Soybean Cotyledonary-node Agrobacterium-mediated Transformation System - http://www.biotech.unl.edu/transgenic/protocols.html

Category: Plant Biology Protocols

Protocol includes: Seed Sterilization Step; Seed Germination Step; Agrobacterium Inoculum Preparation; Explant Preparation; Co-cultivation Plates; Shoot Initiation Step; Shoot Elongation Step; Rooting Step. - [Read Soybean Cotyledonary-node Agrobacterium-mediated Transformation System]

Spore Germination Protocol - http://www-rcf.usc.edu/~forsburg/spores.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for spore germination. This procedure is typically used for the isolation and preparation of spores from a diploid strain heterozygous for a marked disruption (e.g., yfg1::his3+) Inoculation of the spore population into minimal medium lacking the nutritional supplement corresponding to the disruption marker (e.g., minimal medium lacking histidine) allows only the disruption spores to germinate. - [Read Spore Germination Protocol]

Squash Preparation of Plant Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/prosquash.htm

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol for the preparation of plant chromosomes using squash. - [Read Squash Preparation of Plant Chromosomes Protocol]

Squash Preparation of Plant Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/prosquash.htm

Category: Cytogenetics Protocols

Protocol for squash preparation of plant chromosomes. - [Read Squash Preparation of Plant Chromosomes Protocol]

Steps in Hybridoma Production Protocol - http://herpesvirus.tripod.com/research/HYBRIDOM.htm

Category: Antibody Protocols: Antibody Production Protocols

Protocol for steps in hybridoma production. Includes: SIMPLE METHOD: BY FROM GIBCO the HAT and HT concentrate; INSTRUMENTS FOR SPLENECTOMY OF ONE MICE; PREPARATION FOR CELL FUSION; CELL FUSION; SCREENING THE CLONES; RECLONING; ASCITIC FLUID PRODUCTION; PREPARATION OF PERITONEAL MACROPHAGES. - [Read Steps in Hybridoma Production Protocol]

Steroid Radioimmunoassay Protocol - http://faculty.washington.edu/jwingfie/protocols.htm

Category: Immunology: Immunoassays

Protocol for steroid radioimmunoassay. Includes: SOLVENT DISTILLATION; PREPARATION OF PLASMA SAMPLES; EXTRACTION OF STEROIDS AND COLUMN PACKING; COLUMN CHROMATOGRAPHY; RADIOIMMUNOASSAY; SEPARATION OF BOUND AND FREE COUNTS; DIRECT ASSAYS; SHORT COLUMN CHROMATOGRAPHY. - [Read Steroid Radioimmunoassay Protocol]

Storage of Chromosome Preparations for FISH Protocol - http://www.riedlab.nci.nih.gov/publications/2415.3738_Storage_Chrom_Prep.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the storage of chromosome preparations for FISH. Includes: Cell Pellet; Chromosome Slide Preparation. - [Read Storage of Chromosome Preparations for FISH Protocol]

Successfully culturing Schwann cells from adult peripheral nerve - http://www.physiol.usyd.edu.au/daved/papers/1998/sc_culture/

Category: Neuroscience: Neuron Cell Culture Protocols

Preparation of cultures, Ansselin et al., 1998. Acta Chururgica Austriaca 30. - [Read Successfully culturing Schwann cells from adult peripheral nerve]

Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3869

Category: PCR Protocols: cDNA Synthesis Protocols

Protocol describes the preparation of subtracted cDNA probes by hybridization to an mRNA driver, followed by purification of the single-stranded radiolabeled cDNA by hydroxyapatite chromatography. Before preparing the probe, it is a good idea to have filters (which contain the cDNA library to be screened) ready to hybridize. - [Read Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer Protocol]

Tail DNA Preparation Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/TailDNA.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Protocol for Tail DNA preparation. - [Read Tail DNA Preparation Protocol]

TEM Sample Preparation Links - http://bama.ua.edu/~hsmithso/class/bsc_656/websites/sample_tem.html

Category: Molecular Imaging: Electron Microscopy Protocols

Embedding. Diagnostic Electron Microscopy on Reembedded ("Popped Off") Areas of Large Spurr Epoxy Sections ï¿½ LR White Microwave Processing Protocol . TEM Sample Preparation Links and Protocols. - [Read TEM Sample Preparation Links]

Articles (1-10 of 13)

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.