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Preparation of dsRNA Molecules for RNAi in Mouse Oocytes and Early Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4508

Category: RNAi Technology Protocols: RNAi Mouse Embryos and Oocytes Protocols

Protocol describes in vitro transcription with the SP6 polymerase. For other polymerases (T3, T7), simply use the appropriate buffer and desired RNA polymerase instead of the SP6 polymerase. - [Read Preparation of dsRNA Molecules for RNAi in Mouse Oocytes and Early Embryos Protocol]

Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF. - http://www.its.caltech.edu/%7Ebjorker/Prep_of_electocomp_cells.pdf

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF. Bjorkman Group, California I.T. - [Read Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF.]

Preparation of Electrocompetent E. coli DH5 Protocol - http://www.its.caltech.edu/~bjorker/Prep_of_electocomp_cells.pdf

Category: Transfection Protocols: Electroporation Sonoporation Protocols

Protocol for the preparation of electrocompetent E. coli (i.e. DH5 ). - [Read Preparation of Electrocompetent E. coli DH5 Protocol]

Preparation of Electrocompetent E. coli Protocol - http://www.its.caltech.edu/~bjorker/Prep_of_electocomp_cells.pdf

Category: E Coli Protocols: E coli Transformation Protocols

Protocol for the preparation of electrocompetent E. coli. - [Read Preparation of Electrocompetent E. coli Protocol]

Preparation of Electrolyte Gradient Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3795

Category: Nucleic Acid Electrophoresis Protocols

Protocol for the preparation of electrolyte gradient gels. Electrolyte gradients are formed when buffers of different concentrations are used in the upper (low electrolyte concentration) and lower (high electrolyte concentration) chambers of the electrophoresis device. - [Read Preparation of Electrolyte Gradient Gels Protocol]

Preparation of Embryonic Stem (ES) Cells for Injection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4416

Category: Stem Cell Protocols: Stem Cell Injection Protocols

Injection of ES cells into the cavity of blastocysts is one of the main methods for generating chimeras. This protocol describes the preparation of ES cells for injection. - [Read Preparation of Embryonic Stem (ES) Cells for Injection Protocol]

Preparation of Endothelial Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E538DDD4A302A0DF2CDDD9363072C1CA&objectid=6673B460F50B90057960CE47ABB708A4

Category: Cell Biology and Cell Culture: Endothelial Cell Protocols

Endothelial cells, which line blood vessels, can be prepared from a variety of tissues. They are frequently prepared from the umbilical vein, which is relatively easy to obtain. The procedure is clearly described and provides a large population of highly purified endothelial cells. There are also methods for obtaining endothelial cells from other tissues such as fat, skin, and mucosa. These methods require special care and generate smaller populations of cells. - [Read Preparation of Endothelial Cells Protocol]

Preparation of Feeder Layers from Primary Embryonic Fibroblasts Protocol - http://escore.im.wustl.edu/htmldocs/mefs.htm

Category: Stem Cell Protocols: Feeder Cell Preparation Protocols

Protocol for the preparation of feeder layers from primary embryonic fibroblasts. - [Read Preparation of Feeder Layers from Primary Embryonic Fibroblasts Protocol]

Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining - http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Urchin/Urchin_fix.html

Category: Developmental Biology: Sea Urchin Developmental Biology

Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining. Cebra-Thomas. Swathmore. - [Read Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining]

Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html

Category: Nucleic Acid Detection Protocols

Protocol for preparation of fluorescent DNA probe from HUMAN mRNA or total RNA using direct incorporation. Includes: Preparing fluoresenctly labeled cDNA (probe); Washing and Scanning Arrays. - [Read Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation]

Preparation of Fluorescent DNA Probe from HUMAN mRNA/ Total RNA using Direct Incorporation Protocol - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for the preparation of fluorescent DNA probe from HUMAN mRNA or total RNA using direct incorporation. Includes: Preparing fluoresenctly labeled cDNA (probe); Washing and Scanning Arrays. - [Read Preparation of Fluorescent DNA Probe from HUMAN mRNA/ Total RNA using Direct Incorporation Protocol]

Preparation of Frozen Stocks of RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000180.pdf?pid=PP00000180

Category: Cell Culture Protocols: Cell Lines Protocols

Protocol for the preparation of frozen stocks of RAW 264.7 cells. Includes: Freezing procedure and reagents. - [Read Preparation of Frozen Stocks of RAW 264.7 Cells]

Preparation of Genomic DNA from Bacteria Protocol - http://userpages.umbc.edu/~jwolf/m1.htm#genomic%20DNA

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for preparation of genomic DNA from bacteria. - [Read Preparation of Genomic DNA from Bacteria Protocol]

Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4038

Category: Mouse Protocols: Mouse Genetics Protocols

Method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs. - [Read Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol]

Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4038

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Simple protocol is used to extract DNA from small numbers of cultured cells and from fragments of soft or bony tissues. The method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs. - [Read Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol]

Preparation of Genomic DNA from Yeast Using Glass Beads Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4151

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the preparation of genomic DNA from a 5-ml liquid culture of yeast cells. - [Read Preparation of Genomic DNA from Yeast Using Glass Beads Protocol]

Preparation of High Titer Adenovirus in P11 Cells Protocol - http://www.whitelabs.org/Lab%20Protocols/adenovirus%20protocols/Preparation%20of%20High%20Titer%20Adenovirus%20in%20P11%20cells.htm

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for the preparation of high titer adenovirus in P11 cells. - [Read Preparation of High Titer Adenovirus in P11 Cells Protocol]

Preparation of KC Nuclear Extract for In Vitro Splicing - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D34E3CBZRBQMFZR3FQLMCFEWHUWBNSIV0?id=p521

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for preparation of KC nuclear extract for in vitro splicing. Protocol makes 3.4 ml of extract for every 4 liter of cells (depending on initial cell concentration). Protocol includes: Procedure, Solutions, BioReagents and Chemicals and protocol hints. - [Read Preparation of KC Nuclear Extract for In Vitro Splicing]

Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda} Lysogens - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3769

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

In this protocol, a bacterial lysogen is constructed from a recombinant bacteriophage {lambda} encoding a fusion protein of interest. The resulting lysogenic colonies are induced to synthesize the fusion protein, which is then isolated in preparation for functional and biochemical analyses. - [Read Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda} Lysogens]

Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infection - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3771

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

This rapid method is used to screen bacteriophage {lambda}gt11 clones for the production of immunodetectable fusion proteins. After optimizng the conditions of infection and induction, the method can be used to produce preparative amounts of a fusion protein. - [Read Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infection]

Preparation of Messenger-Dependent Rabbit Reticulocyte Lysate - http://www.bio.com/protocolstools/protocol.jhtml?id=p9003

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for preparation of messenger-dependent rabbit reticulocyte lysate. Informamtion also added to test that the lysate is working before doing the Nuclease inactivation - [Read Preparation of Messenger-Dependent Rabbit Reticulocyte Lysate]

Preparation of metaphase chromosomes from lymphoblastoid cell lines - http://www.sanger.ac.uk/HGP/methods/cytogenetics/metaphase.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Preparation of metaphase chromosomes from lymphoblastoid cell lines for FISH analysis. Sanger Institue. - [Read Preparation of metaphase chromosomes from lymphoblastoid cell lines]

Preparation of Metaphase Spread Protocol - http://www.jax.org/staff/mills/protocols/preparation_of_metaphase.pdf

Category: Cytogenetics Protocols

Protocol for the preparation of metaphase spread. Includes three methods. - [Read Preparation of Metaphase Spread Protocol]

Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4483

Category: Stem Cell Protocols: Feeder Cell Preparation Protocols

MEF feeders are prepared weekly to provide a substrate for undifferentiated embryonic stem (ES) cells. Primary MEF cells are thawed, established in culture, treated with mitomycin C to halt their proliferation so they cannot overgrow the ES cultures, and then replated onto dishes convenient for ES cell culture. This protocol can also be used to prepare feeder cells from STO fibroblast cell lines. - [Read Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Plates Protocol]

Preparation of Mouse Tail DNA for Dot Blots or PCR - http://www.muridae.com/wmc/docs/tailDNA_prep.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Tail dots blot, quicker than doing southerns. Muridae.com, Eric Mercer - [Read Preparation of Mouse Tail DNA for Dot Blots or PCR]

Articles (1-10 of 13)

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.