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Preparation of Bacterial Growth Broths and Agar, Antibiotics and IPTG - http://sbio.uct.ac.za/Sbio/documentation/Bacterial%20Media%20and%20solutions.html

Category: Buffers Media, and Solutions: Bacterial Solutions

Preparation of Bacterial Growth Broths and Agar, Antibiotics and IPTG. South African Stuctural Biology Initiative. - [Read Preparation of Bacterial Growth Broths and Agar, Antibiotics and IPTG]

Preparation of Bacteriophage lambda DNA Cleaved with a Single Restriction Enzyme Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3974

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Bacteriophage {lambda} vectors that allow genetic selection of recombinant bacteriophages (e.g., the EMBL series, {lambda}2001, {lambda}DASH, {lambda}ZAP, and {lambda}gt10) can be prepared for cloning by simple digestion by one or more restriction enzymes. - [Read Preparation of Bacteriophage lambda DNA Cleaved with a Single Restriction Enzyme Protocol]

Preparation of Bacteriophage lambda DNA Cleaved with Two Restriction Enzymes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3987

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Many replacement vectors (e.g., the EMBL series, {lambda}2001, and {lambda}DASH) contain a series of restriction sites, arranged in opposite orientations, at each end of the central stuffer fragment. Digestion of these vectors with two different restriction enzymes yields left and right arms, a stuffer fragment, and short segments of the polycloning sites. These can easily be removed from the arms by differential precipitation with isopropanol or spun-column chromatography. - [Read Preparation of Bacteriophage lambda DNA Cleaved with Two Restriction Enzymes Protocol]

Preparation of bOG-DOPG Mixed Micelles Protocol - http://www.duke.edu/web/ceramide/protocols/0027.html

Category: Lipid Protocols

Protocol for the preparation of bDOG-DOPG mixed micelles. Includes: Recrystallizing BOG; Preparing mixed micelles. - [Read Preparation of bOG-DOPG Mixed Micelles Protocol]

Preparation of Brain Membrane Fractions for Western Blot Analysis - http://www.bdbiosciences.com/pharmingen/protocols/Brain_Membrane_Fractions.shtml

Category: Protein Protocols: Western Blot Protocol

Western blotting using enriched membrane preparation and postsynaptic densities are desired. (BD Biosciences) - [Read Preparation of Brain Membrane Fractions for Western Blot Analysis]

Preparation of Broth and Plates - http://www.people.virginia.edu/~gwl6s/protocols/recipes.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Terrific Broth, LB plates, NZY Plates, LB Broth. Laurie Lab, Univ. Virginia Health Science Center. - [Read Preparation of Broth and Plates]

Preparation of C. elegans Genomic DNA Protocol - http://www.genetics.wustl.edu/tslab/Protocols/genomic_DNA_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for preparation of C. elegans genomic DNA. Includes: Solutions; Worm preparation; DNA preparation. - [Read Preparation of C. elegans Genomic DNA Protocol]

Preparation of cell lysates from yeast by enzymatic digestion of the cell wall - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_yeast_enzyme.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast by enzymatic digestion of the cell wall. - [Read Preparation of cell lysates from yeast by enzymatic digestion of the cell wall]

Preparation of cell lysates from yeast using a French Press - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_yeast_press.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast using a French Press. he Protein Expression and Purification Facility, EMBL - [Read Preparation of cell lysates from yeast using a French Press]

Preparation of cell lysates from yeast using glass beads vortexing - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_glass_beads.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast using glass beads vortexing. Includes lysis buffer recipes. The Protein Expression and Purification Facility, EMBL - [Read Preparation of cell lysates from yeast using glass beads vortexing]

Preparation of Cells and Reagents for Flow Cytometry Protocols - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C3CC685613447CEC768412FDE0F&objectid=6674EA50F5C3D9A0189B376C7A89BF6B

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

A procedure for direct and indirect staining of single-cell suspensions of lymphoid tissue or peripheral blood lymphocytes to detect cell surface membrane antigens is presented. In addition, support protocols present methods for fluorescence labeling of purified antibodies. A protocol for flow cytometric analysis of intracellular antigens in single-cell suspensions is also included. - [Read Preparation of Cells and Reagents for Flow Cytometry Protocols]

Preparation of Chromosome Spreads - http://www.le.ac.uk/bl/phh4/prochrom.htm

Category: Cytogenetics Protocols

An ideal method of tissue preparation ensures both good specimen morphology and that the target molecules are in the optimum state for probe access and hybridization. DNA:DNA in situ hybridization is usually carried out on chromosome spread preparations where chromosome and nuclei are released from cells and spread on a glass microscope slide. This method yields well separated and enlarged chromosomes with good morphology which can be analyzed in transmitted light or fluorescence microscopes. - [Read Preparation of Chromosome Spreads]

Preparation of Competent Bacterial Cells - http://www.cbs.umn.edu/~amundsen/chlamy/methods/competent.html

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Simple E.coli competent cell Protocol. Based on a protocol from Neil Olszewski and Scott Medberry.. Craig D. Amundsen - [Read Preparation of Competent Bacterial Cells]

Preparation of Crude Subcellular Fractions by Differential Centrifugation Protocol - http://www.axis-shield.com/densityhome/optiprep/S07.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols

The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient. This protocol describes the use of differential centrifugation to fractionate a mammalian liver homogenate but similar methods should be applicable to all mammalian tissues and cultured cells. - [Read Preparation of Crude Subcellular Fractions by Differential Centrifugation Protocol]

Preparation of cytospin from single cell suspension - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/CytospinPreparation.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Preparation of cytospin from single cell suspension. Columbia University. Cattoretti Lab. - [Read Preparation of cytospin from single cell suspension]

Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3807

Category: DNA Protocols: DNA Sequencing Protocols

The inclusion of formamide in sequencing gels eliminates secondarystructure in the DNA during electrophoresis. Formamide gelsare particularly useful and almost a necessity when sequencingDNA templates with a G/C content >55%. - [Read Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol]

Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3807

Category: Nucleic Acid Electrophoresis Protocols

Protocol for preparation of denaturing polyacrylamide gels containing formamide.The inclusion of formamide in sequencing gels eliminates secondary structure in the DNA during electrophoresis. Formamide gels are particularly useful and almost a necessity when sequencing DNA templates with a G/C content >55%. - [Read Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol]

Preparation of Denaturing Polyacrylamide Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3793

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols

Protocol for preparation of denaturing polyacrylamide gels. - [Read Preparation of Denaturing Polyacrylamide Gels Protocol]

Preparation of Dialysis Tubing - http://mullinslab.ucsf.edu/protocols/html/Preparation_of_Dialysis_tubing.htm

Category: Protein Protocols: Protein Dialysis Protocols

Preparation of Dialysis Tubing. Mullins Lab. UCSF - [Read Preparation of Dialysis Tubing]

Preparation of Dialysis Tubing Protocol - http://ylwang.umassmed.edu/protocol/pp/dialysis.htm

Category: Protein Protocols: Protein Dialysis Protocols

Preparation of Dialysis Tubing. Yu-Li Wang Lab - [Read Preparation of Dialysis Tubing Protocol]

Preparation of DNA Affinity Resin - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4205

Category: Chromatography Protocols

This protocol describes the preparation of concatamerized oligonucleotides and their coupling to cyanogen-bromide-activated Sepharose. The procedure uses a commercially activated resin, which can be purchased as a lyophilized powder. Keith Brocklehurst et al. - [Read Preparation of DNA Affinity Resin - Subscription Required]

Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of DNA from Mammalian Cells - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4030

Category: Nucleic Acid Electrophoresis Protocols: Pulsed Field Gel Electrophoresis Protocols

Protocol for preparation of DNA for pulsed-field gel electrophoresis: isolation of DNA from mammalian cells and tissues. Genomic DNAs from mammalian cells are prepared for pulsed-field gel electrophoresis by lysing cells in situ in an agarose plug. Following digestion with an appropriate restriction enzyme, the plug is loaded directly into the well of a pulsed-field gel or it can be melted before loading. - [Read Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of DNA from Mammalian Cells]

Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of Intact DNA from Yeast - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3235

Category: Nucleic Acid Electrophoresis Protocols: Pulsed Field Gel Electrophoresis Protocols

Protocol for preparation of DNA for pulsed-field gel electrophoresis: isolation of intact DNA from yeast. Yeast cells are first treated enzymatically to break down the cell walls and then resuspended in low-melting-temperature agarose plugs. The DNA is liberated by infusing the plugs with lysis buffer and proteases. This method is used to prepare both conventional and artificial yeast chromosomes. - [Read Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of Intact DNA from Yeast]

Preparation of DNA from Cells in 24-Well Tissue Culture Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4414

Category: Stem Cell Protocols

This protocol describes a method of DNA preparation from ES cells in 24-well plates. - [Read Preparation of DNA from Cells in 24-Well Tissue Culture Plates Protocol]

Preparation of Double-stranded (Replicative Form) Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3281

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

The double-stranded replicative form (RF) of bacteriophage M13 is isolated from infected cells using methods similar to those used to purify plasmid DNA. Several micrograms of RF DNA can be isolated from a 1-2-ml culture of infected cells. - [Read Preparation of Double-stranded (Replicative Form) Bacteriophage M13 DNA Protocol]

Articles (1-10 of 13)

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.