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Competent cell preparation - http://www.chem.uga.edu/scottgrp/GrpProtocols/Competent_cell_preparation.htm

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Bacterial E.Coli competent cell preparation. Calcium Chloride Protocol, Preparation of calcium chloride competent cells for frozen storage, Electroporation Protocol, Electroporation Protocol for transformations using double-stranded plasmids, Reference: - [Read Competent cell preparation]

Antibiotic solutions - http://molbiol.ru/eng/protocol/01_05.html

Category: Microbiology: Antibiotics Concentration in Media LB

Working concentrations and stock solutions for antibiotics, preparation of stock solutions for Ampicillin, Kanamycin and others. Practical Molecular Biology - [Read Antibiotic solutions]

Competent Cell Preparation [CaCl2] - http://www.cf.ac.uk/biosi/staff/kille/Methods/Gene/Comp_cell_CaCl.html

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Nice protocol for the preparation of Bacterial cells to allow uptake of plasmid vectors. Dr Peter Kille. Hot Metals Cardiff. - [Read Competent Cell Preparation [CaCl2]]

Agar Plates for Selection of Clones in Bacteria - http://www.le.ac.uk/biology/phh4/bactplte.htm

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

LB Agar: Luria Broth, X-Gal preparation, IPTG and ampicillin. Petri dish preparation. Pat Heslop-Harrison and Trude Schwarzacher, Univ. Leicester - [Read Agar Plates for Selection of Clones in Bacteria]

Agarose Gel Preparation Protocol - http://fp.bio.utk.edu/mycology/Techniques/mt-agarose_gels.htm

Category: Molecular Biology Protocols: Agarose Gel Preparation

Solutions for Agarose gel prepartion, Selection of Appropriate Gel Size and Composition. Mycology Lab University of Tennessee - [Read Agarose Gel Preparation Protocol]

Preparation of ultra-competent E. coli cells for transformation - http://www.hybtech.org/Liu/uccell.html

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Preparation of ultra-competent E. coli cells for transformation. Dr. Chun-Ming Liu. Based on Inoue et al (1990). - [Read Preparation of ultra-competent E. coli cells for transformation]

Agarose Gel Electrophoresis PDF - http://www.uni-tuebingen.de/evoeco/files/methods/Electrophoresis.pdf

Category: Molecular Biology Protocols: Agarose Gel Preparation

Agarose Gel Electrophoresis. Pouring of Agarose Gel, Preparation of samples, Gel electrophoresis, Ethidium bromide staining. - [Read Agarose Gel Electrophoresis PDF]

Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots - http://faculty.virginia.edu/mirmira/resources_files/Protocols/Nuclear%20Extracts.htm

Category: Protein Protocols: Nuclear Extraction Protocols

Nuclear Extract protocol is optimized for about 10^7 cells, can use for gel shift assays and western blotting. Mirmira Lab, Univ. Virginia - [Read Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots]

Protein Dialysis Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p9054

Category: Protein Protocols: Protein Dialysis Protocols

Protein Dialysis Protocol. Bioprotocol. Preparation of Dialysis Tubing, Use of the Dialysis Tubing and Dialysis buffer. - [Read Protein Dialysis Protocol]

ELISA Method - http://mycoplasmas.vm.iastate.edu/lab_site/methods/immunol/ELISA.html

Category: ELISA Protocols

Reagents, protocol and Conjugate Preparation. Minion Lab, College of Veterinary Medicine, Iowa State. - [Read ELISA Method]

Method: Preparation of Competent Cells - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid13.html

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Detailed protocol for creating bacterial competent cells preparation. Matthew S. Holt - [Read Method: Preparation of Competent Cells]

Cell Staining for Immunofluorescence Microscopy - http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml

Category: Molecular Imaging: Immunofluorescence Microscopy

Cell Staining for Immunofluorescence Microscopy. Includes protocols for fixing the cells, Coverslip Preparation for Adherent Cells, Coverslip Preparation for Non-Adherent Cells, Paraformaldehyde Fixation, and Methanol/Acetone Fixation. Blocking protocols include blocking with primary antibody, and incubation with secondary antibody. - [Read Cell Staining for Immunofluorescence Microscopy]

AFLP protocol - http://www.msu.edu/user/hazensam/aflp/AFLPprotocolMSU.html

Category: PCR Protocols: AFLP PCR

Restriction Digestion of Genomic DNA , Adapter preparation, Ligation of Adapters. Pre amplification Reactions. Gel electrophoresis, Silver Staining. AFLP Protocol and procedure. - [Read AFLP protocol]

Cell Lysate Extracts - http://www.upstate.com/misc/protocol_detail.q.prot.e.CellLysate_Prep.a.name.e.Cell_Lysate_Extracts-General_Protocols

Category: Protein Protocols: Protein Extraction From Cells

Cell Lysate Extracts. Great protocols for cell lysis preparation from a variety of cell types. There are numerous methods of cell stimulation and lysis. For a given protein, Upstateâ€™s Laboratories determine the specific treatment upon initial testing of its products. It is important to select the correct cell line, stimulation procedure (if any), and lysis protocol. Upstate. - [Read Cell Lysate Extracts]

Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF. - http://www.its.caltech.edu/%7Ebjorker/Prep_of_electocomp_cells.pdf

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF. Bjorkman Group, California I.T. - [Read Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF.]

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) - http://www.mcb.uct.ac.za//sdspage.htm

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE). Ed Rybicki and Maud Purves. Dept Microbiology, University of Cape Town. PROTOCOL includes: Assembling Gel Apparatus, Resolving Gels, Stacking Gels, Electrophoresis buffer, Sample Preparation for Sds-page and Staining of Gels. - [Read SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)]

Preparation of Broth and Plates - http://www.people.virginia.edu/~gwl6s/protocols/recipes.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Terrific Broth, LB plates, NZY Plates, LB Broth. Laurie Lab, Univ. Virginia Health Science Center. - [Read Preparation of Broth and Plates]

Western Blot Detailed (PDF) - http://www.its.caltech.edu/%7Ebjorker/Western_blot.pdf

Category: Protein Protocols: Western Blot Protocol

Protocol includes cell lysate preparation, SDS-PAGE gels, transfer, and blotting. (Bjorkman Group). - [Read Western Blot Detailed (PDF)]

Antibiotics. and Antibiotics Stock Calculator Tools - http://molbiol.edu.ru/eng/protocol/01_05.html

Category: Microbiology: Antibiotics Concentration in Media LB

Working concentrations and stock solutions and which antibiotics are light sensitive. Preparation of stock solutions and calculator tool to calculate concentrations for stock solutions. Practical Molecular Biology. - [Read Antibiotics. and Antibiotics Stock Calculator Tools]

Antibiotics - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Materials/antibiotics.html

Category: Microbiology: Antibiotics Concentration in Media LB

Antibiotic stock solutions preparation, Storage conditions for antibiotics, Working Concentrations for high copy plasmids. Kay Schneitz Univ Zurich. - [Read Antibiotics]

Large scale nuclear extract preparation - http://mesentsev.narod.ru/Biblioteque/Hattori.html

Category: Protein Protocols: Nuclear Extraction Protocols

Homogenize cells or tissue, Pellet nuclei, Lyse nuclei, Reprecipitate nuclear proteins, dialyze nuclear extract. Hattori M et al., DNA Cell Biol. 1990. Homogenization buffer and dialysis buffer recipes. - [Read Large scale nuclear extract preparation]

Universal Mouse Genotyping Protocol Using PCR - http://mgc.wustl.edu/protocols/pcr_genotyping.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Universal Mouse Genotyping Protocol, Primer Design, PCR Cycling, HotSHOT Preparation of Murine DNA for PCR. WUSM. Mouse Genetics Core. - [Read Universal Mouse Genotyping Protocol Using PCR]

DNA Preparation from Blood PDF - http://www.riedlab.nci.nih.gov/publications/2438.2413_DNA_Prep_Blood.pdf

Category: DNA Protocols: DNA Extraction Blood

Method for DNA Preparation from Blood. Using lysis buffer and phenol/chloroform/isoamyl alcohol. Thomas Ried, NCI. - [Read DNA Preparation from Blood PDF]

Preparing Chemically Competent Cells - http://openwetware.org/wiki/Preparing_chemically_competent_cells

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Detailed protocol Preparation and Steps for E.Coli Competent Cells procedure. Open Net Ware. - [Read Preparing Chemically Competent Cells]

Preparation of Competent Bacterial Cells - http://www.cbs.umn.edu/~amundsen/chlamy/methods/competent.html

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Simple E.coli competent cell Protocol. Based on a protocol from Neil Olszewski and Scott Medberry.. Craig D. Amundsen - [Read Preparation of Competent Bacterial Cells]

Articles (1-10 of 13)

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.