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Neuron Culture Solutions - http://www.ehlerslab.org/protocols.html

Category: Neuroscience: Neuron Cell Culture Protocols

Neuron Cell Culture Solutions, Neuron Culturing, Preparation of Brain Membrane Fractions, Biotinylation Assay of Receptor Endocytosis, PSD Prep. Ehlers lab Duke Univ. - [Read Neuron Culture Solutions]

Other MALDI-TOF Sample Prep Protocols - http://www.hmc.psu.edu/core/oldsite/Maldi/othermalditofprotocols.htm

Category: Proteomic Protocols

Other MALDI-TOF Sample Prep Protocols. BA Stanley. - [Read Other MALDI-TOF Sample Prep Protocols]

Phenol/Freeze RNA Prep Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/rprep.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol for phenol/freeze RNA prep. - [Read Phenol/Freeze RNA Prep Protocol]

Plasmid Midi-Prep from Bacteria Protocol - http://ivaan.com/protocols/101.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for plasmid midi-prep from bacteria. - [Read Plasmid Midi-Prep from Bacteria Protocol]

Porcine Brain Tubulin Prep Protocol - http://www.bio.unc.edu/faculty/salmon/lab/protocolsporcinetubulin.html

Category: Cytoskeleton Protocols: Microtubules Protocols

The protocol calls for 3 pig brains, and should yield ~ 60 mg of purified tubulin. - [Read Porcine Brain Tubulin Prep Protocol]

Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol - http://www.fgsc.net/neurosporaprotocols/VM%20vacuole%20procedure.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa (4 liter prep, easily scaled up to 10, 12, 24, or 30 l). - [Read Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol]

Quick DNA Plasmid Prep Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D4.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for quick DNA plasmid prep. Protocol gives very clean plasmid preps for restriction digests and cloning. However, due to the alkaline lysis step, the DNA is often nicked and may not give exceptional sequence data. - [Read Quick DNA Plasmid Prep Protocol]

Quick DNA Plasmid Prep. Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D2.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for quick DNA plasmid prep. This is a very fast mini-prep protocol which is suitable for sequence analysis and restriction digests. - [Read Quick DNA Plasmid Prep. Protocol]

Simple Mini-prep Protocol - http://irc.igd.cornell.edu/Protocols/Mini-prep.htm

Category: Microbiology: Mini-Prep Plasmid Purification

Simple Mini-prep Protocol, using Chloroform/Isoamyl alcohol (IAA). Cornell. - [Read Simple Mini-prep Protocol]

Single Strand DNA Prep for Sequencing Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S2.html

Category: DNA Protocols: DNA Sequencing Protocols

Protocol for single strand DNA prep for sequencing. Protocol works well from a 5 ml starting culture or a 1 ml starting culture. - [Read Single Strand DNA Prep for Sequencing Protocol]

Single Strand DNA Prep For Sequencing Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S2.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for single strand DNA prep for sequencing. This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly). - [Read Single Strand DNA Prep For Sequencing Protocol]

Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html

Category: DNA Protocols: TOPO Cloning

Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]

Yeast Chromosomal DNA Prep Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/chromosomal-DNA.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast chromosomal DNA prep. - [Read Yeast Chromosomal DNA Prep Protocol]

Articles (1-10 of 25)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.