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Alkaline Lysis Mini-Prep Protocol - http://preuss.bsd.uchicago.edu/protocols/Alkaline.html

Category: Microbiology: Mini-Prep Plasmid Purification

Harvesting and Lysis of Bacteria. Mini-prep plasmid purification using Solution I, Solution II and Solution III. The Preuss Lab. Univ. Chicago. - [Read Alkaline Lysis Mini-Prep Protocol]

Boiling Protein Prep Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/Protein/proteinprep.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Preparation of protein extracts for western blot. - [Read Boiling Protein Prep Protocol]

C. elegans Genomic DNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/genomic_dna_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans genomic DNA prep. - [Read C. elegans Genomic DNA Prep Protocol]

C. elegans mRNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/joes_mrna_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans mRNA prep. Includes: Oligo-dT cellulose prep; mRNA prep. - [Read C. elegans mRNA Prep Protocol]

C. elegans RNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/yale_rna_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans RNA prep. - [Read C. elegans RNA Prep Protocol]

C. elegans RNAi Protocol - http://www.zoology.ubc.ca/~alorch/rnai-protocol.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans RNAi. Includes: Transformation of competent cells; Blunt-end ligation; Preparation of competent cells; Dephosphorylation of linear plasmid DNA; Restriction Digest: EcoRV; Insert amplification from gDNA; Gel purification: QiaQuick gel purification kit; Mini-prep; Transformant Screening; Bacterial preparation and induction; Preparation of worms for RNAi feeding. - [Read C. elegans RNAi Protocol]

cDNA from Frozen Tissue Sections and LCM - http://dir.nichd.nih.gov/lcm/prep.htm

Category: RNA Protocols: RT-PCR and cDNA synthesis

Method of synthesizing cDNA from Frozen Tissue Sections Protocol. Includes LCM - Laser Capture Microdissection Protocols. NIH - [Read cDNA from Frozen Tissue Sections and LCM]

CHO Centrosome Prep Protocol - http://mitchison.med.harvard.edu/protocols/mt1.html

Category: Cell Organelle Protocols: Nucleus Protocols

The key step is the lysis which solubilizes centrosomes away from nuclei by very low ionic strength lysis after treatment of cells with nocodazole and cytochalasin B. The released centrosomes are then centrifuged onto a Ficoll cushion (to avoid pelleting) and the interface between the lysate and the Ficoll is collected and the centrosomes are concentrated on a sucrose gradient. Fractions are assayed by spindown and double IF with 5051 serum and anti-tubulin and the pooled fractions are frozen... - [Read CHO Centrosome Prep Protocol]

Column Method for Lambda Phage DNA Preparation - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda11.html

Category: Molecular Biology Protocols: Phage Protocols and Methods: Lambda Phage Protocols

Column Method for Mini-prep of lambda phage DNA purification from lysates. Donis Keller Lab - [Read Column Method for Lambda Phage DNA Preparation]

Column Method for Lambda Phage DNA Preparation Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda11.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Mini-prep method for lambda phage DNA purification from lysates. - [Read Column Method for Lambda Phage DNA Preparation Protocol]

CsCl Prep of Plasmid DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for CsCl prep of plasmid DNA. This is a standard large scale prep. For plasmid DNA which gives a yield of 0.5 1.0 mg. - [Read CsCl Prep of Plasmid DNA Protocol]

CSF Extract Prep for Xenopus Spindle Assembly Protocol - http://mitchison.med.harvard.edu/protocols/ext3.html

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for CSF extract prep for Xenopus spindle assembly. - [Read CSF Extract Prep for Xenopus Spindle Assembly Protocol]

Dauer Pheromone Prep Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=12

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for Dauer pheromone prep. Includes small molecule prep and cleaner prep. - [Read Dauer Pheromone Prep Protocol]

DNA Plasmid Prep. Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for DNA plasmid prep. - [Read DNA Plasmid Prep. Protocol]

DNA Prep for CGH Protocol - http://www.genomics.princeton.edu/dunham/MDyeastDNAprep.htm

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for DNA prep for CGH. - [Read DNA Prep for CGH Protocol]

Drosophila Fly Food Prep Method Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/method_press_n_seal.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila fly food prep method. - [Read Drosophila Fly Food Prep Method Protocol]

Drosophila Single Fly Genomic DNA Prep Protocol - http://vosshall.rockefeller.edu/protocols/SingleFlyGenomic.pdf

Category: Drosophila Protocols: Drosophila Nucleic Acids Protocols

Protocol for Drosophila single fly genomic DNA prep. - [Read Drosophila Single Fly Genomic DNA Prep Protocol]

Fungal DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml). - [Read Fungal DNA Isolation Protocol]

GST Fusion Protein Prep - http://cmgm.stanford.edu/~vesicle/protocols/gst_prot.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Detailed protocols and methods for GST protein purification. Vesicle Trafficking, Stanford. - [Read GST Fusion Protein Prep]

Harvesting for RNA - http://www.genomics.princeton.edu/dunham/MDyeastRNA.htm

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Harvesting for RNA. Includes: RNA prep; Small RNA prep; Large RNA prep. - [Read Harvesting for RNA]

High Molecular Weight Yeast Liquid DNA Preparation Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast4.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol that isolates intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. Using this protocol one can expect a yield of 100-200 µg of DNA per prep. - [Read High Molecular Weight Yeast Liquid DNA Preparation Protocol]

Live Cell Prep for Sorting Protocol - http://depts.washington.edu/immunweb/cellanalysis/Sortprp.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Protocol for live cell prep for sorting. - [Read Live Cell Prep for Sorting Protocol]

Mini-Prep Protocol - http://www.udel.edu/Biology/fschmieg/midiprep411.htm

Category: Microbiology: Mini-Prep Plasmid Purification

Mini-prep Protocol. Florence Schmieg. University of Delaware. - [Read Mini-Prep Protocol]

Mini-Prep Protocol - http://ivaan.com/protocols/107.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for mini-prep. Includes: PREPARATION OF E. COLI; PRODUCTION OF CLEAR LYSATE; cDNA ISOLATION. - [Read Mini-Prep Protocol]

Mini-Prep Protocol for Purification of Plasmid DNA from Bacteria - http://people.morehead-st.edu/fs/d.peyton/protocols.html

Category: Microbiology: Mini-Prep Plasmid Purification

Using Solutions A for Lysis, Solutions B for Neutralization Solution, and Solution C for washing. Dr.David Peyton. - [Read Mini-Prep Protocol for Purification of Plasmid DNA from Bacteria]

Articles (1-10 of 25)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.